Chemical properties of type 1 and type 2 periglomerular cells in the mouse olfactory bulb are different from those in the rat olfactory bulb
Chemical properties of type 1 and type 2 periglomerular cells in the mouse olfactory bulb are different from those in the rat olfactory bulb
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DOI：
10.1016/j.brainres.2007.04.087
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发表时间：
2007-09-05
期刊：
影响因子：
2.9
通讯作者：
Kosaka, Toshio
中科院分区：
文献类型：
--
作者：
Kosaka, Katsuko;Kosaka, Toshio
我们分析了C57B/6J品系小鼠主嗅球肾小球旁区域的细胞组成，重点关注1)肾小球的区室组织和1型和2型肾小球周围细胞的存在，2)4种主要化学鉴定的肾小球周围细胞群，谷氨酸脱羧酶(GAD)/ γ -氨基丁酸（GABA），酪氨酸羟化酶，calretinin和calbindin D28k阳性肾小球周围细胞之间的共定位关系。3)一氧化氮合酶（NOS）阳性肾小球旁细胞的化学性质。我们证实了小鼠肾小球的室状组织以及肾小球周围1型和2型细胞的存在。与大鼠肾小球周围细胞相似，酪氨酸羟化酶阳性细胞为1型，GAD/ gaba阳性。另一方面，calbindin d28k阳性和calretinin阳性细胞均为2型肾小球周围细胞，但与GAD/ gaba阴性的大鼠相比，所有calbindin d28k阳性的肾小球周围细胞和65%的calbindin阳性的肾小球周围细胞均为GAD/ gaba阳性。因此，GAD/ gaba阳性细胞包括1型和2型肾小球周围细胞。nos阳性细胞被认为是大鼠肾小球周围1型细胞的一个亚群，它与calretinin阳性和calbindin d28k阳性细胞分离。然而，在小鼠中，约70%的nos阳性细胞为calretinin阳性，50%的calretinin阳性细胞为nos阳性。我们在此揭示了肾小球周围细胞化学性质的显著物种差异，并表明小鼠主嗅球的细胞组织不能从大鼠的细胞组织中推断出来。(c) 2007 Elsevier B.V.版权所有
We analyzed the cellular composition of the juxtaglomerular region in the main olfactory bulb of C57B/6J strain mice, focusing on 1) the compartmental organization of the glomerulus and the presence of type 1 and 2 periglomerular cells, 2) the colocalization relationships among the 4 major chemically identified groups of periglomerular cells, glutamic acid decarboxylase (GAD)/gamma-aminobutyric acid (GABA), tyrosine hydroxylase, calretinin and calbindin D28k positive periglomerular cells, and 3) the chemical properties of the nitric oxide synthase (NOS)-positive juxtaglomerular cells. We confirmed the compartmental organization of the glomerulus and the presence of both type 1 and 2 periglomerular cells in the mice. Similar to rat periglomerular cells, the tyrosine hydroxylase -positive cells were type 1 and GAD/GABA-positive. On the other hand, both the calbindin D28k-positive and calretinin-positive cells were type 2 periglomerular cells, but in contrast to those in rats, which are GAD/GABA-negative, all of the calbindin D28k-positive periglomerular cells and 65% of the calretinin -positive periglomerular cells were GAD/GABA-positive. The GAD/GABA-positive cells thus included both type 1 and type 2 periglomerular cells. Juxtaglomerular NOS-positive cells have been proposed as a subgroup of type 1 periglomerular cells that are separate from the calretinin-positive and calbindin D28k-positive cells in rats. However, in the mice, about 70% of the NOS-positive cells were calretinin-positive, and 50% of the calretinin -positive cells were NOS-positive. We herein reveal the significant species differences in the chemical properties of periglomerular cells and suggest that the cellular organization of the mouse main olfactory bulb cannot be extrapolated from that of rats. (c) 2007 Elsevier B.V. All rights reserved.