FYVE1 Is Essential for Vacuole Biogenesis and Intracellular Trafficking in Arabidopsis
FYVE1 Is Essential for Vacuole Biogenesis and Intracellular Trafficking in Arabidopsis
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DOI：
10.1104/pp.114.253377
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发表时间：
2015-04-01
期刊：
影响因子：
7.4
通讯作者：
Isono, Erika
中科院分区：
文献类型：
--
作者：
Kolb, Cornelia;Nagel, Marie-Kristin;Isono, Erika
植物液泡是植物生活史中参与各种生物学过程的中心细胞器。因此，阐明液泡的生物发生和维持机制是我们理解这些过程的基础。液泡的正常形成依赖于细胞内膜运输途径。虽然在过去已经发现了几个液泡形态发生改变的突变体，但是植物液泡发生的分子基础还没有完全阐明。为了确定液泡生物发生所必需的关键因素，我们在拟南芥（Arabidopsis thaliana）中进行了正向遗传学筛选，并分离出液泡形态改变的突变体。液泡融合缺陷1（vfd 1）突变体表现出幼苗致死性和中央液泡形成缺陷。VFD 1编码一个含有Fab 1、YOTB、Vac 1和EEA 1（FYVE）结构域的蛋白质FYVE 1，该蛋白质参与细胞内运输。FYVE 1定位于晚期内体上，并与含Src同源性-3结构域的蛋白相互作用。FYVE 1的突变体在泛素介导的蛋白质降解、空泡运输和自噬方面有缺陷。总之，我们的研究结果表明，FYVE 1是必不可少的植物生长和发育和地方FYVE 1作为一个关键的调节细胞内运输和液泡生物发生。
The plant vacuole is a central organelle that is involved in various biological processes throughout the plant life cycle. Elucidating the mechanism of vacuole biogenesis and maintenance is thus the basis for our understanding of these processes. Proper formation of the vacuole has been shown to depend on the intracellular membrane trafficking pathway. Although several mutants with altered vacuole morphology have been characterized in the past, the molecular basis for plant vacuole biogenesis has yet to be fully elucidated. With the aim to identify key factors that are essential for vacuole biogenesis, we performed a forward genetics screen in Arabidopsis (Arabidopsis thaliana) and isolated mutants with altered vacuole morphology. The vacuolar fusion defective1 (vfd1) mutant shows seedling lethality and defects in central vacuole formation. VFD1 encodes a Fab1, YOTB, Vac1, and EEA1 (FYVE) domain-containing protein, FYVE1, that has been implicated in intracellular trafficking. FYVE1 localizes on late endosomes and interacts with Src homology-3 domain-containing proteins. Mutants of FYVE1 are defective in ubiquitin-mediated protein degradation, vacuolar transport, and autophagy. Altogether, our results show that FYVE1 is essential for plant growth and development and place FYVE1 as a key regulator of intracellular trafficking and vacuole biogenesis.