Student Collaboration in a Series of Integrated Experiments To Study Enzyme Reactor Modeling with Immobilized Cell-Based Invertase
Student Collaboration in a Series of Integrated Experiments To Study Enzyme Reactor Modeling with Immobilized Cell-Based Invertase
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学生合作进行一系列综合实验，研究固定化细胞转化酶的酶反应器模型
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Susana B. Santos
中科院分区：
文献类型：
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作者：
M. Taipa;A. Azevedo;António Grilo;Pedro T. Couto;F. Ferreira;A. Fortuna;I. Pinto;R. Santos;Susana B. Santos
介绍了酶催化的基本原理及其在反应器操作和建模中的应用。倒置酶是一种催化蔗糖水解的β-果糖呋喃苷酶，在最佳条件下（pH 4.5和45°C）作为模型酶。实验工作包括每个学生每周3小时的实验时间，为期四周。学生分成三组，每组3或4名学生，以协作的方式工作，使用含有生物活性转化酶的游离和海藻酸钙固定化的bayanus Saccharomyces细胞，获得不同底物浓度下初始反应速率的一组重复。研究结果由所有研究组共享，用于统计数据处理，Michaelis-Menten动力学参数的计算，以及三种经典反应器的建模，连续搅拌槽反应器（CSTR），塞流反应器（PFR）和流化床反应器（FBR）。
An integrative laboratory study addressing fundamentals of enzyme catalysis and their application to reactors operation and modeling is presented. Invertase, a β-fructofuranosidase that catalyses the hydrolysis of sucrose, is used as the model enzyme at optimal conditions (pH 4.5 and 45 °C). The experimental work involves 3 h of laboratory time for each student per week over four weeks. Students, organized in laboratory sessions of three groups with 3 or 4 students per group, work in a collaborative manner to obtain a set of replicates of initial reaction rate for different substrate concentrations, using both free and calcium alginate-immobilized Saccharomyces bayanus cells containing biologically active invertase. The results are shared by all of the groups for statistical data treatment, calculation of Michaelis–Menten kinetic parameters, and modeling of three types of classical reactors, a Continuous Stirred Tank Reactor (CSTR), a Plug Flow Reactor (PFR), and a Fluidized Bed Reactor (FBR), operating i...