Prevalence, abundance, serovars and antimicrobial resistance of Salmonella isolated from retail raw poultry meat in China
Prevalence, abundance, serovars and antimicrobial resistance of Salmonella isolated from retail raw poultry meat in China
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中国零售生禽肉中沙门氏菌的流行率、丰度、血清型和耐药性
DOI：
10.1016/j.scitotenv.2019.136385
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发表时间：
2020-04-15
影响因子：
9.8
通讯作者：
Wu, Qingping
中科院分区：
文献类型：
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作者：
Yang, Xiaojuan;Huang, Jiahui;Wu, Qingping
本研究调查了中国零售生禽肉中沙门氏菌污染的流行情况和水平，并检查了回收菌株的血清型分布和抗菌药物敏感性。从中国39个城市的零售市场共采集了664份禽肉样品。从249份（37.5%）样品中分离出沙门氏菌，其中鸡190份（36.7%），鸭48份（40.7%），鸽11份（39.2%）。36.1%的阳性样品的最大可能数（MPN）值范围为0.3至10 MPN/g，其中3个样品超过110 MPN/g。在667株沙门氏菌中，确定了35个血清型和42个多位点序列分型模式。主要血清型包括肠道沙门氏菌肠道血清型（32.7%）、肠道沙门氏菌印第安纳州血清型（142%）和肠道沙门氏菌鼠伤寒血清型（11.9%），同时发现了两种新型ST（ST 7352和ST 7612）。除了一个未命名的菌株（4，12：d：-）外，所有已鉴定的血清型先前都与人类感染有关。对318株非重复分离株的药敏试验显示，只有5株（1.6%）对所有22种供试抗生素敏感，而191株（60.1%）对至少3种抗生素表现出耐药性。耐药水平最高的是萘啶酸（723%），其次是氨苄西林（55.3%）和链霉素（48.7%）。特别值得关注的是检测到高度多重耐药的印第安纳州肠道沙门氏菌血清型分离株，其中大多数（84.1%）对环丙沙星和头孢曲松表现出共同耐药。总体而言，我们的研究结果显示，零售生禽肉中沙门氏菌污染的流行率很高，这可能使消费者暴露于多重耐药菌株。本研究为评估家禽沙门氏菌污染的新控制措施提供了全面的数据。(C)2020 Elsevier B. V.保留所有权利。
This study investigated the prevalence and levels of Salmonella contamination of retail raw poultry meat in China, and examined serovar distribution and antimicrobial susceptibility profiles of the recovered isolates. In total, 664 poultry meat samples were collected from retail markets in 39 cities across China. Salmonella was isolated from 249 (375%) samples, including 190 (36.7%) chicken, 48 (40.7%) duck and 11 (392%) pigeon samples. The most probable number (MPN) values of 36.1% of the positive samples ranged from 0.3 to 10 MPN/g, with three samples exceeding 110 MPN/g. Among the 667 Salmonella isolates, 35 serovars and 42 multilocus sequence typing patterns were identified. Predominant serovars included Salmonella enterica serovar Enteritidis (32.7%), Salmonella enterica serovar Indiana (142%) and Salmonella enterica serovar Typhimurium (11.9%), while two novel STs were identified (ST7352 and ST7612). Except for one unnamed strain (4,12:d:-), all of the identified serovars have previously been linked to human infections. Antimicrobial susceptibility testing of the 318 non-duplicate isolates revealed that only 5 (1.6%) were susceptible to all 22 tested antimicrobials, while 191 (60.1%) exhibited resistance to at least three dasses of antimicrobials. The highest levels of resistance were observed for nalidixic add (723%), followed by ampicillin (55.3%) and streptomycin (48.7%). Of particular concern was the detection of highly multidrug-resistant Salmonella enterica serovar Indiana isolates, most (84.1%) of which showed co-resistance to ciprofloxacin and ceftriaxone. Overall, our findings showed a high prevalence of Salmonella contamination of retail raw poultry meat, which could expose consumers to multidrug-resistant isolates. This study provides comprehensive data for evaluation of new control measures for Salmonella contamination of poultry. (C) 2020 Elsevier B.V. All rights reserved.