INVOLVEMENT OF RHO IN GTP-GAMMA-S-INDUCED ENHANCEMENT OF PHOSPHORYLATION OF 20 KDA MYOSIN LIGHT-CHAIN IN VASCULAR SMOOTH-MUSCLE CELLS - INHIBITION OF PHOSPHATASE-ACTIVITY
INVOLVEMENT OF RHO IN GTP-GAMMA-S-INDUCED ENHANCEMENT OF PHOSPHORYLATION OF 20 KDA MYOSIN LIGHT-CHAIN IN VASCULAR SMOOTH-MUSCLE CELLS - INHIBITION OF PHOSPHATASE-ACTIVITY
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DOI：
10.1016/0014-5793(95)00573-r
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发表时间：
1995-07-03
期刊：
影响因子：
3.5
通讯作者：
TAKUWA, Y
中科院分区：
文献类型：
--
作者：
NODA, M;YASUDAFUKAZAWA, C;TAKUWA, Y
在β-七叶皂苷透化的培养的猪主动脉平滑肌细胞中，GTP γ S剂量依赖性地增强20 kDa肌球蛋白轻链（MLC（20））的Ca 2+诱导的渥曼青霉素敏感性磷酸化，GTP γ S不增强MLC的硫代磷酸化（20），但抑制其去磷酸化。其特异性地使ADP-核糖基化并使小分子量G蛋白的rho家族失活，完全消除GTP γ S的作用，这些结果表明，Rite参与了GTP γ S诱导的主动脉平滑肌细胞中Ca 2+依赖性MLC（20）磷酸化的增强，并强烈表明rho的这种作用是由于对MLC的蛋白磷酸酶活性的抑制（20）。
In beta-escin-permeabilized cultured pig aortic smooth muscle cells GTP gamma S dose-dependently enhances Ca2+-induced, wortmannin-sensitive phosphorylation of 20 kDa myosin light chain (MLC(20)), GTP gamma S does not potentiate thiophosphorylation of MLC(20), but does inhibit its dephosphorylation, Pretreatment with C, botulinum exotoxin C-3, which specifically ADP-ribosylates and inactivates the rho family of the small molecular weight G proteins, completely abolishes the effects of GTP gamma S, These results indicate that rite is involved in the GTP gamma S-induced enhancement of Ca2+-dependent MLC(20) phosphorylation in aortic smooth muscle cells, and strongly suggest that this effect of rho is due to inhibition of protein phosphatase activity toward MLC(20).