Variable low-mass filtering using an electrodynamic ion funnel.
Variable low-mass filtering using an electrodynamic ion funnel.
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DOI：
10.1002/jms.900
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发表时间：
2005-09
期刊：
影响因子：
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通讯作者：
J. Page;A. Tolmachev;Keqi Tang;Richard D. Smith
中科院分区：
文献类型：
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作者：
J. Page;A. Tolmachev;Keqi Tang;Richard D. Smith
为电喷雾电离（ESI）源开发了一种可调节的低质量过滤器，以阻止与不需要的背景物质相关的离子进入质谱仪。低质量过滤器通过使用来自电动离子漏斗的电导限制板的可调节势能势垒制成，其禁止具有较高离子迁移率的物质离开ESI源。我们表明，这种安排提供了一个线性的电压调节低质量过滤从m/z 0至500。也进行高于m/z 500的质量过滤;然而，更高质量的物质被衰减。用牛血清白蛋白（BSA）胰蛋白酶消化物的液相色谱/质谱（LC/MS）分析测试了质量过滤器，结果表明其能够阻断低质量背景物质，在峰洗脱和检测期间，其占ESI源后面总离子电流的40-70%。
An adjustable low-mass filter has been developed for an electrospray ionization (ESI) source to block ions associated with unwanted background species from entering the mass spectrometer. The low-mass filter is made by using an adjustable potential energy barrier from the conductance-limiting plate of an electrodynamic ion funnel, which prohibits species with higher ion mobilities from exiting the ESI source. We show that this arrangement provides a linear voltage adjustment for low-mass filtering from m/z 0 to 500. Mass filtering above m/z 500 is also performed; however, higher-mass species are attenuated. The mass filter was tested with a liquid chromatography/mass spectrometry (LC/MS) analysis of a bovine serum albumin (BSA) tryptic digest and resulted in the ability to block low-mass, background species, which accounted for 40-70% of the total ion current immediately behind the ESI source during peak elution and detection.