How to measure first-order hepatic transfer coefficients by distributed modeling of a recirculating rat liver perfusion system.
How to measure first-order hepatic transfer coefficients by distributed modeling of a recirculating rat liver perfusion system.
复制标题
如何通过再循环大鼠肝脏灌注系统的分布式建模来测量一阶肝传输系数。
DOI：
10.1152/ajpgi.1982.243.6.g518
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Forker,EL
中科院分区：
文献类型：
--
作者：
Luxon,BA;King,PD;Forker,EL
用数字计算机模拟了大鼠肝脏灌流系统储罐中溶质消失曲线的演化过程。结果表明，仅利用正弦流的分布和非交换血管系统的传递函数的近似信息就可以很好地估计这些动力学参数。相比之下，对摄取和排出(但不是排泄)的转移系数的令人满意的估计在很大程度上取决于对系统转运时间的准确测量。一种适合于实施该方法的灌流系统被测试并发现能够维持正常的肝功能60分钟以上。讨论了实现所需体积和流量测量的方法，并提出了识别信息量太低而不能得到可靠结果的消失曲线的经验判据。
A digital computer is used to simulate solute disappearance curves evolving in the reservoir of a rat liver perfusion system. The results show that it is feasible to obtain good estimates of these kinetic parameters with only approximate information about the distribution of sinusoidal flows and the transfer function of the nonexchanging vasculature. By contrast satisfactory estimates of the transfer coefficients for uptake and efflux (but not excretion) are critically dependent on accurate measurements of the system transit times. A perfusion system suitable for implementing the method is tested and found to maintain normal liver function for upward of 60 min. The means to achieve the required volume and flow measurements are discussed, and an empirical criterion is developed for recognizing disappearance curves whose information content is too low to yield reliable results.