Distinct Roles of Ventromedial versus Ventrolateral Striatal Medium Spiny Neurons in Reward-Oriented Behavior.
Distinct Roles of Ventromedial versus Ventrolateral Striatal Medium Spiny Neurons in Reward-Oriented Behavior.
复制标题
腹内侧与腹外侧纹状体中棘神经元在奖励导向行为中的不同作用。
DOI：
10.1016/j.cub.2017.08.061
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发表时间：
2017
期刊：
影响因子：
9.2
通讯作者：
Tanaka KF.
中科院分区：
文献类型：
--
作者：
Tsutsui-Kimura I;Natsubori A;Mori M;Kobayashi K;Drew MR;de Kerchove d'Exaerde A;Mimura M;Tanaka KF.
腹侧纹状体（VS）是调节奖励导向行为的关键大脑中心[1 - 4]。根据皮层输入模式，VS在解剖学上可分为内侧（VMS）和外侧（VLS）部分。VMS接收来自内侧苍白球起源的边缘结构（例如，内侧前额叶皮质[mPFC]），VLS接收来自外侧苍白球起源区域的输入（例如，（5）[6]。这种解剖学特征使我们假设VS在调节奖励导向行为方面存在功能隔离。在这里，我们设计了一个纤维光度测定系统[4]，并在食物寻找辨别任务期间监测多巴胺D2受体表达的中型棘神经元（D2-MSN）的群体水平Ca2+活动，D2-MSN是纹状体中的主要细胞类型之一。我们发现，VLS D2-MSNs被激活的时候，线索介绍。与此形成鲜明对比的是，VMS D2-MSN在该时间点被抑制。这些变化在VLS和VMS的光遗传抵消受损的行动启动和增加对非奖励线索的反应，分别。在反向按压训练期间，提示呈现时的VMS抑制暂时停止，并且VMS D2-MSNs的光遗传学激活促进了新的偶然性的获得。这些数据表明，VMS和VLS中的抑制和兴奋对奖励导向行为中选择和启动适当的行动是重要的。我们提出了不同的次区域内的VS在执行成功的奖励导向的行为的作用。
The ventral striatum (VS) is a key brain center regulating reward-oriented behavior [1–4]. The VS can be anatomically divided into medial (VMS) and lateral (VLS) portions based on cortical input patterns. The VMS receives inputs from medial pallium-originated limbic structures (e.g., the medial prefrontal cortex [mPFC]), and the VLS receives inputs from the lateral pallium-originated areas (e.g., the insula) [5, 6]. This anatomical feature led us to hypothesize a functional segregation within the VS in terms of the regulation of reward-oriented behavior. Here, we engineered a fiber photometry system [4] and monitored population-level Ca2+activities of dopamine D2-receptor-expressing medium spiny neurons (D2-MSNs), one of the major cell types in the striatum, during a food-seeking discrimination task. We found that VLS D2-MSNs were activated at the time of cue presentation. In stark contrast, VMS D2-MSNs were inhibited at this time point. Optogenetic counteraction of those changes in the VLS and VMS impaired action initiation and increased responding toward non-rewarded cues, respectively. During lever-press reversal training, VMS inhibition at the time of cue presentation temporarily ceased and optogenetic activation of VMS D2-MSNs facilitated acquisition of the new contingency. These data indicate that the opposing inhibition and excitation in VMS and VLS are important for selecting and initiating a proper action in a reward-oriented behavior. We propose distinct subregional roles within the VS in the execution of successful reward-oriented behavior.