Generation of dynamic temporal and spatial concentration gradients using microfluidic devices
Generation of dynamic temporal and spatial concentration gradients using microfluidic devices
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DOI：
10.1039/b313600k
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发表时间：
2004-01-01
期刊：
影响因子：
6.1
通讯作者：
Jeon, NL
中科院分区：
文献类型：
--
作者：
Lin, F;Saadi, W;Jeon, NL
本文描述了一种使用单个微流体装置生成动态时间和空间浓度梯度的微流体方法。与之前描述的为每个设备生成单个固定梯度形状的方法相比，该方法将简单的“混合器模块”与梯度生成网络相结合，以控制和操纵许多不同的梯度形状。梯度分布由流体输入的配置以及微通道网络的设计决定。通过使用单独的注射泵控制流体输入的相对流速，可以动态控制供给梯度发生器的入口的组成，以生成时间和空间梯度。为了演示该概念并说明该方法，显示了生成（1）均匀浓度的时间梯度，（2）具有动态控制斜率、基线和方向的线性梯度，以及（3）具有受控非线性的非线性梯度的设备示例，并描述了它们的局限性。
This paper describes a microfluidic approach to generate dynamic temporal and spatial concentration gradients using a single microfluidic device. Compared to a previously described method that produced a single fixed gradient shape for each device, this approach combines a simple "mixer module" with gradient generating network to control and manipulate a number of different gradient shapes. The gradient profile is determined by the configuration of fluidic inputs as well as the design of microchannel network. By controlling the relative flow rates of the fluidic inputs using separate syringe pumps, the resulting composition of the inlets that feed the gradient generator can be dynamically controlled to generate temporal and spatial gradients. To demonstrate the concept and illustrate this approach, examples of devices that generate ( 1) temporal gradients of homogeneous concentrations, ( 2) linear gradients with dynamically controlled slope, baseline, and direction, and (3) nonlinear gradients with controlled nonlinearity are shown and their limitations are described.