Mie-type scattering and non-Beer-Lambert absorption behavior of human cells in infrared microspectroscopy
Mie-type scattering and non-Beer-Lambert absorption behavior of human cells in infrared microspectroscopy
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DOI：
10.1529/biophysj.104.057950
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发表时间：
2005-05-01
影响因子：
3.4
通讯作者：
Woody, BR
中科院分区：
文献类型：
--
作者：
Mohlenhoff, B;Romeo, M;Woody, BR
我们报告的红外显微光谱特征的细胞核在一个完全不活跃和收缩（固缩）状态，并积极分裂细胞的细胞核。对于固缩核，非常高的DNA局部浓度导致染色质不透明，因此，在非常小的核的IR光谱中没有DNA信号。然而，这些核可以通过它们的散射特性来检测，这可以通过介质球散射的米氏理论来描述。这种散射取决于细胞核的大小，因此，计算和观察固缩和有丝分裂的细胞核有很大不同的散射截面。
We report infrared microspectral features of nuclei in a completely inactive and contracted ( pyknotic) state, and of nuclei of actively dividing cells. For pyknotic nuclei, the very high local concentration of DNA leads to opaqueness of the chromatin and, consequently, the absence of DNA signals in the IR spectra of very small nuclei. However, these nuclei can be detected by their scattering properties, which can be described by the Mie theory of scattering from dielectric spheres. This scattering depends on the size of the nucleus; consequently, quite different scattering cross-sections are calculated and observed for pyknotic and mitotic nuclei.