Dual mode bioreactions on polymer nanoparticles covered with phosphorylcholine group
Dual mode bioreactions on polymer nanoparticles covered with phosphorylcholine group
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DOI：
10.1016/j.colsurfb.2006.04.006
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发表时间：
2006-06-01
影响因子：
5.8
通讯作者：
Ishihara, Kazuhiko
中科院分区：
文献类型：
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作者：
Ito, Tomomi;Watanabe, Junji;Ishihara, Kazuhiko
我们研究了磷酸胆碱（PC）基团覆盖的聚合物纳米粒子的制备和蛋白质的固定化，以观察纳米粒子上的双模式生物反应。为了对纳米颗粒进行表面修饰，合成了一种以PC基团作为亲水部分的水溶性两亲性磷脂聚合物。在这种聚合物中，引入了一个活性酯基，它可以使蛋白质变性。以磷脂聚合物为增溶剂，采用溶剂挥发法从二氯甲烷溶液中制备了聚L-乳酸纳米粒。纳米颗粒的直径约为100 nm。200 nm处，表面覆盖有PC基团和活性酯基。通过蛋白质中的活性酯基与氨基之间的反应，蛋白质可以在温和的条件下吸附在纳米粒子上。将抗体和酶都固定在纳米颗粒上，并观察到诸如抗原/抗体反应和酶反应的生物反应。当抗原被添加到纳米颗粒的悬浮液中时，纳米颗粒发生聚集，然后它们沉淀。此外，当将酶底物加入到悬浮液中时，酶促反应进行得很好。基于这些结果，我们提供了用抗体和酶两者官能化的聚合物纳米颗粒，并且可以发生双模式生物反应。我们的结论是，新的聚合物纳米粒子可以用于纳米/微米尺度的诊断和医疗系统。(C)2006年由Elsevier B. V.出版
We investigated the preparation of polymer nanoparticles covered with phosphorylcholine (PC) groups and the immobilization of proteins in order to observe dual mode bioreactions on the nanoparticles. For the Surface modification on the nanoparticles, a water-soluble amphiphilic phospholipid polymer with PC groups as a hydrophilic moiety was synthesized. In this polymer, an active ester group, which can immobilize proteins, was introduced. Using the phospholipid polymer as a solubilizer, poly(L-lactic acid) nanoparticles were prepared from its methylene chloride solution in an aqueous medium by the solvent evaporation method. The diameter of the nanoparticles was ca. 200 nm and the surface was covered with the PC groups and active ester groups. Proteins Could immobilize on the nanoparticles under mild conditions by the reaction between the active ester group and amino group in the proteins. Both an antibody and enzyme were immobilized on the nanoparticles and bioreactions such as the antigen/antibody reaction and enzymatic reaction were observed. When an antigen was added to the suspension of the nanoparticles, aggregation of the nanoparticles occurred and then they precipitated. Also, the enzymatic reaction proceeded well when the enzyme substrate was added to the suspension. Based on these results, we provided polymer nanoparticles functionalized with both the antibody and enzyme, and the dual mode bioreactions could occur. We concluded that the novel polymer nanoparticles Could be used for nano-/micro-scaled diagnostic and medical treatment systems. (C) 2006 Published by Elsevier B.V.