Fluorometer for endoscopic diagnosis of tumors.
Fluorometer for endoscopic diagnosis of tumors.
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DOI：
10.1118/1.595521
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发表时间：
1984-07
期刊：
影响因子：
3.8
通讯作者：
A. Profio;D. Doiron;J. Sarnaik
中科院分区：
文献类型：
--
作者：
A. Profio;D. Doiron;J. Sarnaik
已经开发了一种适合于内窥镜应用的滤光荧光计，用于通过先前注射的肿瘤特异性试剂血卟啉衍生物的荧光来检测和表征浅表肿瘤。荧光由通过内窥镜中的光纤光导传导的紫光激发，并且荧光发射与反射的紫光一起由另一光纤光导收集。红色荧光和紫色荧光通过分色镜和滤光片分离，并在光电倍增管中检测。荧光信号与反射的紫色信号的比率与荧光产率与紫色反射率的比率成比例，但对距离、角度和紫色功率的变化不敏感。该仪器可用于定位小肿瘤，并用于定量测量肿瘤中血卟啉衍生物的量，这是光辐射治疗中精确剂量测定的要求。
A filter fluorometer suitable for endoscopic applications has been developed for detection and characterization of superficial tumors by the fluorescence of a previously injected, tumor-specific agent, hematoporphyrin derivative. Fluorescence is excited by violet light conducted through a fiberoptic lightguide in the endoscope, and the fluorescence emission together with reflected violet are collected by another fiberoptic lightguide. The red fluorescence and violet are separated by a dichroic mirror and filter and detected in photomultiplier tubes. The ratio of the fluorescence signal to the reflected violet signal is proportional to the ratio of the fluorescence yield to the violet reflectivity but is insensitive to variations in distance, angle, and violet power. The instrument may be useful for localizing small tumors, and for quantitative measurements of the amount of hematoporphyrin derivative in the tumor, a requirement for accurate dosimetry in photoradiation therapy.