Mu Opioid Receptor from the Zebrafish Exhibits Functional Characteristics as Those of Mammalian Mu Opioid Receptor
Mu Opioid Receptor from the Zebrafish Exhibits Functional Characteristics as Those of Mammalian Mu Opioid Receptor
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DOI：
10.1089/zeb.2009.0594
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发表时间：
2009-09-01
期刊：
影响因子：
2
通讯作者：
Rodriguez, Raquel E.
中科院分区：
文献类型：
--
作者：
Fdez. de Velasco, Ezequiel Marron;Law, Ping Y.;Rodriguez, Raquel E.
本文报道了斑马鱼μ阿片受体（zfMOR）的功能特性。在HEK-293细胞系中转染后，在竞争和饱和结合实验中使用肽能和非肽能阿片配体，除了S-3 '-5-GTPgS结合测定和细胞内3'-5 '-环磷酸腺苷（cAMP）水平测定的功能测定外，我们证明zf莫尔表现出与哺乳动物莫尔相似的药理学特征。此外，在阿片激动剂处理（吗啡、DAMGO、埃托啡）后zfMOR的内化过程类似于针对其哺乳动物对应物观察到的模式。这些相似性表明，斑马鱼是研究阿片类药物在发育中作用的良好模型。
The functional characterization of the mu opioid receptor from the zebrafish (zfMOR) is reported here. After transfection in HEK-293 cell line, using both peptidergic and nonpeptidergic opioid ligands in the competition and saturation-binding experiments, in addition to the functional assays of S-35-GTPgS-binding assays and intracellular 3'-5'-cyclic adenosine monophosphate (cAMP) level determinations, we demonstrate that zfMOR exhibits a pharmacological profile similar to that of the mammalian MOR. Besides, the internalization process of zfMOR after opiate agonist treatment (morphine, DAMGO, etorphine) resembles the pattern observed for its mammalian counterpart. These similarities suggest that the zebrafish is a good model for the study of the opioid effects in development.