Protein Interactions and Disease Phenotypes in the ABC Transporter Superfamily
Protein Interactions and Disease Phenotypes in the ABC Transporter Superfamily
复制标题
ABC 转运蛋白超家族中的蛋白质相互作用和疾病表型
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
A. Sali
中科院分区：
文献类型：
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作者：
Libusha Kelly;R. Karchin;A. Sali
ABC transporter proteins couple the energy of ATP binding and hydrolysis to substrate transport across a membrane. In humans, clinical studies have implicated mutations in 19 of the 48 known ABC transporters in diseases such as cystic fibrosis and adrenoleukodystrophy. Although divergent in sequence space, the overall topology of these proteins, consisting of two transmembrane domains and two ATP-binding cassettes, is likely to be conserved across diverse organisms. We examine known intra-transporter domain interfaces using crystallographic structures of isolated and complexed domains in ABC transporter proteins and find that the nucleotide binding domain interfaces are better conserved than interfaces at the transmembrane domains. We then apply this analysis to identify known disease-associated point and deletion mutants for which disruption of domain-domain interfaces might indicate the mechanism of disease. Finally, we suggest a possible interaction site based on conservation of sequence and disease-association of point mutants.
影响因子：
16
作者：
Smith, PC;Karpowich, N;Hunt, JF
通讯作者：
Hunt, JF
影响因子：
16
作者：
Chen, J;Lu, G;Quiocho, FA
通讯作者：
Quiocho, FA