Engineered electrospun polycaprolactone (PCL)/octacalcium phosphate (OCP) scaffold for bone tissue engineering
Engineered electrospun polycaprolactone (PCL)/octacalcium phosphate (OCP) scaffold for bone tissue engineering
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DOI：
10.1016/j.msec.2017.07.041
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发表时间：
2017-12-01
影响因子：
7.9
通讯作者：
Afarani, Mandi Shafiee
中科院分区：
文献类型：
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作者：
Heydari, Zohre;Mohebbi-Kalhori, Davod;Afarani, Mandi Shafiee
骨组织工程的主要挑战是寻找合适的生物替代物作为支架材料。因此，在这项工作中，一种新的支架组成的磷酸八钙（OCP）颗粒共沉淀法制备和聚己内酯（PCL），采用静电纺丝技术。采用SEM、FTIR、XRD、DSC和TGA等分析手段对电纺支架进行了表征。测定了复合支架的最大拉伸应力、断裂应变和杨氏模量等力学性能。采用模拟体液浸泡法测定支架的生物活性。将人成骨细胞G-292接种于支架表面，进行体外细胞培养和MTT法检测。FTIR和XRD结果表明，OCP组分已适当地掺入到聚合物PCL基体中。SEM分析显示，由于OCP，纤维尺寸显著减小。拉伸试验结果表明，PCL/OCP复合材料具有较好的力学性能。此外，OCP颗粒导致在SBF溶液附近的支架表面形成羟基磷灰石层。从MTT测定获得的结果描述了OCP颗粒对成骨细胞人G-292细胞在支架上的生长具有积极影响。总之，PCL/OCP复合支架的上述特征使其成为骨组织工程应用的一个很好的候选者。
The main challenge in bone tissue engineering is to find suitable biological substitutes which act as scaffolds. Hence, in this work, a novel scaffold composed of octacalcium phosphate (OCP) particles fabricated by co precipitation method and polycaprolactone (PCL) using electrospinning technique was introduced. The electrospun scaffolds were characterized by SEM, FTIR, XRD, DSC and TGA analysis. The mechanical properties of the composite scaffolds including maximum tensile stress, strain at break and Young modulus were measured. The bioactivity of the scaffolds was determined by soaking in simulated body fluid (SBF). The osteoblast human G-292 cells were seeded on the scaffold's surface for in vitro studies including cell culture and MTT assay. The FTIR and XRD results showed that OCP component has an appropriate incorporation into the polymeric PCL matrix. The SEM analysis exhibited a significant reduction in the fiber size thanks to the OCP. The results of tensile test confirmed that the PCL/OCP composite introduced suitable mechanical properties. Furthermore, the OCP particles led to form hydroxyapatite layer on the scaffold's surface in the vicinity of SBF solution. The obtained results from the MTT assay described that OCP particles have a positive impact on the growth of the osteoblast human G-292 cells on the scaffolds. Overall, aforesaid features of the PCL/OCP composite scaffold make it a great candidate for the bone tissue engineering application.