Antimicrobial Effect of Titanium Hydroxyapatite in Denture Base Resin
Antimicrobial Effect of Titanium Hydroxyapatite in Denture Base Resin
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DOI：
10.3390/app8060963
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发表时间：
2018-06
期刊：
影响因子：
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通讯作者：
W. Sato;Y. Yoshida;S. Komasa;Y. Hasegawa;J. Okazaki
中科院分区：
文献类型：
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作者：
W. Sato;Y. Yoshida;S. Komasa;Y. Hasegawa;J. Okazaki
在当前的研究中，我们研究了义齿基托树脂中钛羟基磷灰石（TiHA）（一种光氧化有机材料）对实验室细菌形成的单物种生物膜和人类唾液细菌形成的多物种生物膜的抗菌作用。尽管据报道 TiHA 可以限制紫外线 A (UVA) 照射下的浮游细菌的生长，但 TiHA 对细菌生物膜的抗菌作用仍有待阐明。通过将 TiHA 添加到聚甲基丙烯酸甲酯基义齿基托树脂中来制备树脂样本。将样本与形成生物膜的血链球菌、内氏放线菌、金黄色葡萄球菌、大肠杆菌或从志愿者获得的人类唾液中的细菌一起孵育。 UVA 照射后，测定样本上形成的生物膜的菌落形成单位 (CFU)。在含有 TiHA 的样本上形成生物膜的血链球菌、内氏放线菌和金黄色葡萄球菌的 CFU 数量显着低于在不含 TiHA 的样本上形成的生物膜。 TiHA 不会降低形成生物膜的大肠杆菌的 CFU。在所有情况下，唾液细菌在含有 TiHA 的样本上形成的生物膜中的 CFU 数量均显着减少。此外，56小时的UVA照射和28天的水中浸泡均不会减弱TiHA的抗菌效果。义齿基托树脂中的 TiHA 对单一物种细菌生物膜和人类唾液中多种细菌形成的生物膜发挥抗菌作用。
In the current study, we investigated the antimicrobial effect of titanium hydroxyapatite (TiHA), a photo-oxidizing organic material, in denture base resin on single-species biofilms formed by laboratory bacteria and on multispecies biofilms formed by bacteria from the human saliva. Although TiHA reportedly restricts the growth of planktonic bacteria upon ultraviolet A (UVA) irradiation, the antimicrobial effect of TiHA on bacterial biofilms remains to be elucidated. Resin specimens were prepared by adding TiHA to polymethyl methacrylate-based, denture base resin. The specimens were incubated with biofilm-forming Streptococcus sanguinis, Actinomyces naeslundii, Staphylococcus aureus, Escherichia coli, or bacteria from the human saliva obtained from volunteers. After UVA irradiation, the colony-forming units (CFUs) from the biofilms formed on the specimens were determined. CFU numbers for S. sanguinis, A. naeslundii, and S. aureus that formed biofilms on TiHA-containing specimens were significantly lower than those formed on specimens without TiHA. TiHA did not reduce the CFUs of biofilm-forming E. coli. In all cases, CFU numbers in the biofilms formed on TiHA-containing specimens by the salivary bacteria were significantly reduced. In addition, neither a 56 h UVA irradiation nor a 28 d soaking in water diminished the antibacterial effect of TiHA. TiHA in denture base resin exerts an antimicrobial effect on single-species bacterial biofilms and biofilms formed by a wide variety of bacteria from human saliva.