Enzymatic Epoxide-Opening Cascades Catalyzed by a Pair of Epoxide Hydrolases in the Ionophore Polyether Biosynthesis
Enzymatic Epoxide-Opening Cascades Catalyzed by a Pair of Epoxide Hydrolases in the Ionophore Polyether Biosynthesis
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DOI：
10.1021/ol200100e
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发表时间：
2011-04-01
期刊：
影响因子：
5.2
通讯作者：
Oikawa, Hideaki
中科院分区：
文献类型：
--
作者：
Minami, Atsushi;Migita, Akira;Oikawa, Hideaki
我们最近发现的第一个环氧化物水解酶Lsd19，参与拉沙洛西A的生物合成，使我们调查一个长期存在争议的问题，酶促环氧化物开放级联的机制。通过定点突变和结构域分析，揭示了Lsd19催化反应的机理，特别是酸性氨基酸对和催化结构域的作用。
Our recent findings of the first epoxide hydrolase Lsd19, involved in lasalocid A biosynthesis, led us to investigate a long-standing controversial issue on the mechanism of enzymatic epoxide-opening cascades. The site-directed mutagenesis and domain dissection analysis to reveal the mechanism of the reaction catalyzed by Lsd19 is examined, especially in the role of acidic amino acid pair and catalytic domains.