Plasma levels of a novel noncyclooxygenase-derived prostanoid (8-isoprostane) correlate with severity of liver injury in experimental alcoholic liver disease.
Plasma levels of a novel noncyclooxygenase-derived prostanoid (8-isoprostane) correlate with severity of liver injury in experimental alcoholic liver disease.
复制标题
一种新型非环氧合酶衍生的前列腺素（8-异前列腺素）的血浆水平与实验性酒精性肝病中肝损伤的严重程度相关。
DOI：
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发表时间：
1994
影响因子：
3.5
通讯作者：
S. Sadrzadeh
中科院分区：
文献类型：
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作者：
Amin A. Nanji;S. Khwaja;S. Tahan;S. Sadrzadeh
本研究采用灌胃法建立大鼠酒精性肝病模型，探讨酒精性肝病病理严重程度与脂质过氧化反应的关系。通过测量血浆中一种新型非环加氧酶衍生的前列腺素（8-异前列腺素）来评估脂质过氧化。在1个月时处死6组喂食乙醇和不同膳食脂肪（饱和脂肪、玉米油和鱼油）的动物。进行肝脏组织学检查、8-异前列烷的血浆测量和微粒体共轭二烯的测量。喂食鱼油和乙醇的动物出现最严重的肝损伤，血浆中8-异前列烷水平最高（919 +/- 112 pg/ml）。这些水平显著高于玉米油-乙醇组（498 +/-105 pg/ml）（P < 0.02）和饱和脂肪-乙醇组（28.6 +/-11.8 pg/ml）（P <0.001）。喂食饱和脂肪和葡萄糖以及玉米油和葡萄糖的大鼠的水平< 20 pg/ml。然而，喂食鱼油和葡萄糖的大鼠的平均8-异前列烷水平比饱和脂肪-葡萄糖和玉米油-葡萄糖组高约100倍。在鱼油处理组（r = 0.92，P <0.001）和非鱼油处理组（r = 0.94，P <0.001）中观察到病理严重程度和血浆8-异前列烷水平之间的显著相关性。在8-异前列烷水平和肝微粒体共轭二烯之间也观察到显著的相关性（r = 0.93，P <0.001）。我们的研究结果提供了强有力的支持的假设，脂质过氧化作用在乙醇喂养的大鼠病理性肝损伤。
We used the intragastric feeding rat model for alcoholic liver disease to investigate the relationship between pathological severity and lipid peroxidation. Lipid peroxidation was assessed by measurement, in plasma, of a novel noncyclooxygenase-derived prostanoid (8-isoprostane). Six groups of animals fed ethanol and different dietary fats (saturated fat, corn oil and fish oil) were sacrificed at 1 month. Histological liver examination, plasma measurements of 8-isoprostane and measurements of microsomal conjugated dienes were carried out. Animals fed fish oil and ethanol developed the most severe liver injury and had the highest 8-isoprostane levels in plasma (919 +/- 112 pg/ml). These levels were significantly higher than the levels seen in the corn oil-ethanol (498 +/- 105 pg/ml) (P < 0.02) and saturated fat-ethanol (28.6 +/- 11.8 pg/ml) (P < .001) groups. Rats fed saturated fat and dextrose and corn oil and dextrose had levels of < 20 pg/ml. However rats fed fish oil and dextrose had, on average, 8-isoprostane levels about 100-fold higher than those seen in the saturated fat-dextrose and corn oil-dextrose groups. A significant correlation between pathological severity and plasma 8-isoprostane levels was seen in the fish oil (r = 0.92, P < .001) and non-fish oil-treated groups (r = 0.94, P < .001). A significant correlation also was seen between 8-isoprostane levels and liver microsomal conjugated dienes (r = 0.93, P < .001). Our results provide strong support for the hypothesis that lipid peroxidation in ethanol-fed rats contributes to pathological liver injury.