Pasteurisation and Homogenisation of Milk Enhances the Immunogenicity of Milk Plasma Proteins in a Rat Model
Pasteurisation and Homogenisation of Milk Enhances the Immunogenicity of Milk Plasma Proteins in a Rat Model
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DOI：
10.1080/09540109999771
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发表时间：
1999-09
影响因子：
3
通讯作者：
C. Feng;A. Collins
中科院分区：
文献类型：
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作者：
C. Feng;A. Collins
各组大鼠分别腹腔注射0.5 ml原料奶、脱脂奶、巴氏杀菌奶或巴氏杀菌/均质奶进行免疫。在第14天，用相同的制剂增强动物，并在第28天收集血清样本用于检测针对血浆蛋白的IgG抗体。检测样品中酪蛋白、β -乳球蛋白、牛γ球蛋白和牛血清白蛋白的抗体。与用生奶或脱脂奶免疫的动物相比，用巴氏消毒/均质奶免疫的动物血清中检测到的所有特异性抗体浓度都明显更高。用巴氏消毒/均质牛奶免疫组的抗体浓度也高于用巴氏消毒牛奶免疫组，尽管仅在对酪蛋白的反应中观察到显著差异（p < 0.01）。另外的动物用加了锁眼帽贝血青素（KLH）的生奶或巴氏消毒/均质奶进行两次免疫。
Groups of rats were immunised intraperitoneally with 0.5 ml of raw milk, skim milk, pasteurised milk or pasteurised/homogenised milk. On day 14, animals were boosted with identical preparations, and serum samples were collected on day 28 for the measurement of IgG antibodies directed against milk plasma proteins. Samples were assayed for antibodies to casein, beta-lactoglobulin, bovine gamma globulin and bovine serum albumin. Significantly higher concentrations of antibodies of all specificities were detected in the serum of animals immunised with pasteurised/homogenised milk compared with animals immunised with raw or skim milk. Higher concentrations of antibodies were also seen in the group immunised with pasteurised/homogenised milk than the group immunised with pasteurised milk, although significant differences were only seen in the response to casein (p < 0.01). Additional animals were twice immunised with either raw milk fortified with keyhole limpet hemocyanin (KLH), or pasteurised/homogenised milk...