Uric acid quantification in fingernail of gout patients and healthy volunteers using HPLC-UV
Uric acid quantification in fingernail of gout patients and healthy volunteers using HPLC-UV
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使用 HPLC-UV 定量痛风患者和健康志愿者的指甲中的尿酸
DOI：
10.1002/bmc.3687
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发表时间：
2016-08-01
影响因子：
1.8
通讯作者：
Min, Jun Zhe
中科院分区：
文献类型：
--
作者：
Li, Xi-Ling;Shi, Qing;Min, Jun Zhe
尿酸（UA）水平升高是痛风的一个征兆，也就是高尿酸血症。在这项研究中，监测尿酸水平的微创生物样品，如人的指甲，建议痛风的诊断和治疗。26名健康志愿者（HV）和22名痛风患者（GP）进行了研究。从人指甲样品中提取UA，然后在Inertsil ODS-3柱（250 × 4.6 mm i.d.，4.0 m，GL Sciences）通过使用甲醇-74 mm磷酸盐缓冲液（pH 2.2）2：98（v/v）等度洗脱。使用UV检测器在284 nm处监测样品。使用开发的方法，不同的UA浓度被发现在GP和HV。当比较GP与HV的浓度时，观察到UA之间存在统计学显着相关性（p <0.01）。在这项研究中，尿酸被证实是一个潜在的生物标志物的诊断和治疗痛风。我们已经开发了一种新的灵敏，简便的方法来测定尿酸在指甲GP和HV。人类指甲可以作为诊断痛风的非侵入性生物样本。版权所有（c）2016约翰威利父子有限公司
The presence of elevated uric acid (UA) levels is a sign of gout, that is, hyperuricemia. In this study the monitoring of the UA levels in less-invasive biological samples, such as the human fingernail, is suggested for the diagnosis and therapy of gout. Twenty-six healthy volunteers (HV) and 22 gout patients (GP) were studied. The UA was extracted from human fingernail samples, then separated on an Inertsil ODS-3 column (250 x 4.6 mm i.d., 4.0 m, GL Sciences) by isocratic elution using methanol-74 mm phosphate buffer (pH 2.2) 2:98 (v/v). A UV detector was used to monitor the samples at 284 nm. Using the developed method, different UA concentrations were found in the GP and HV. When comparing the concentrations from GP with those from HV, a statistically significant correlation was observed between the UA (p < 0.01). In this study, the UA was confirmed as a potential biomarker for the diagnosis and therapy of gout. We have developed a novel sensitive, and simple method for the determination of UA in the fingernails of GP and HV. The human fingernail may serve as a noninvasive biosample for the diagnosis of gout. Copyright (c) 2016 John Wiley & Sons, Ltd.