Identification of malaria parasites by fluorescence microscopy and acridine orange staining.
Identification of malaria parasites by fluorescence microscopy and acridine orange staining.
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通过荧光显微镜和吖啶橙染色鉴定疟疾寄生虫。
DOI：
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发表时间：
1973
影响因子：
11.1
通讯作者：
T. Sodeman
中科院分区：
文献类型：
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作者：
G. Shute;T. Sodeman
人们普遍认识到，需要一种比使用罗曼诺夫斯基染色的厚血膜更敏感的技术来检测血液中低浓度的疟疾寄生虫。其中一个更有前途的方法似乎是荧光染料染色与吖啶橙子。然而，关于该技术有效性的报告相互矛盾，并且不清楚在调查条件下拍摄的血片在多大程度上会包含可能混淆诊断的荧光伪影。一项调查表明，只要采取合理的谨慎措施，在调查条件下制作的血片几乎不含混淆伪影。然而，人们发现，虽然吖啶橙子染色可能有轻微的优势时，大的疟疾寄生虫的存在，它是劣于常规的Romanowsky染色检测年轻的滋养体，劣势变得更加明显的寄生虫浓度降低。
The need for a technique that is more sensitive than the use of Romanowsky-stained thick blood films for detecting malaria parasites at low concentration in the blood is well recognized. One of the more promising methods appeared to be fluorochrome staining with acridine orange. However, reports on the efficacy of the technique were contradictory and it was not clear to what extent blood films taken under survey conditions would contain fluorescing artefacts that might confuse diagnosis. An investigation indicated that, provided reasonable care was taken, blood films made under survey conditions contained few confusing artefacts. However, it was found that, while acridine orange staining might have a slight advantage when large malaria parasites were present, it was inferior to routine Romanowsky staining for the detection of young trophozoites, the inferiority becoming more pronounced as the parasite concentration decreased.