Comparative mapping of powdery mildew resistance gene Pm21 and functional characterization of resistance-related genes in wheat
Comparative mapping of powdery mildew resistance gene Pm21 and functional characterization of resistance-related genes in wheat
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小麦白粉病抗性基因Pm21的比较定位及抗性相关基因的功能表征
DOI：
10.1007/s00122-016-2668-4
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发表时间：
2016-04-01
影响因子：
5.4
通讯作者：
Bie, Tongde
中科院分区：
文献类型：
--
作者：
He, Huagang;Zhu, Shanying;Bie, Tongde
利用新开发的标记对白粉病抗性基因Pm21进行了物理定位和比较定位。结果表明，Pm21基因是小麦白粉病抗性所必需的候选基因。Pm21基因来源于小麦野生近缘簇毛麦，已转入普通小麦，并在小麦白粉病抗性育种中得到了广泛应用。此前，Pm21已通过使用删除库定位到6VS的Bin Fl0.45-0.58。然而，它的精细测绘仍然是一项艰巨的工作。本研究根据水稻、水稻和小麦基因组的共线性，获得了30个6VS特异的基因标记，并将其物理和比较地定位在Bin FL0.45-0.58及其附近的染色体区域。根据这些图谱，携带Pm21的Bin Fl0.45-0.58与标记6VS-03和6VS-23紧密相连，进一步缩小了Brachypodium和水稻的同源区域，分别为1.06Mb和1.38Mb。在Brachypodium和水稻共有的保守基因中，通过大麦条纹花叶病毒诱导的基因沉默(BSMV-VIGS)和转录模式分析，证实了四个丝氨酸/苏氨酸蛋白激酶基因(DvMPK1、DvMLPK、DvUPK和DvPSYR1)、一个蛋白磷酸酶基因(DvPP2C)和两个转录因子基因(DvGATA和DvWHY)是Pm21介导的小麦白粉病抗性所必需的。综上所述，本研究对Pm21基因座的遗传基础和Pm21介导的抗病途径提供了新的见解。
The powdery mildew resistance gene Pm21 was physically and comparatively mapped by newly developed markers. Seven candidate genes were verified to be required for Pm21 -mediated resistance to wheat powdery mildew.Pm21, a gene derived from wheat wild relative Dasypyrum villosum, has been transferred into common wheat and widely utilized in wheat resistance breeding for powdery mildew. Previously, Pm21 has been located to the bin FL0.45-0.58 of 6VS by using deletion stocks. However, its fine mapping is still a hard work. In the present study, 30 gene-derived 6VS-specific markers were obtained based on the collinearity among genomes of Brachypodium distachyon, Oryza and Triticeae, and then physically and comparatively mapped in the bin FL0.45-0.58 and its nearby chromosome region. According to the maps, the bin FL0.45-0.58 carrying Pm21 was closely flanked by the markers 6VS-03 and 6VS-23, which further narrowed the orthologous regions to 1.06 Mb in Brachypodium and 1.38 Mb in rice, respectively. Among the conserved genes shared by Brachypodium and rice, four serine/threonine protein kinase genes (DvMPK1, DvMLPK, DvUPK and DvPSYR1), one protein phosphatase gene (DvPP2C) and two transcription factor genes (DvGATA and DvWHY) were confirmed to be required for Pm21-mediated resistance to wheat powdery mildew by barley stripe mosaic virus-induced gene silencing (BSMV-VIGS) and transcriptional pattern analyses. In summary, this study gives new insights into the genetic basis of the Pm21 locus and the disease resistance pathways mediated by Pm21.