Cloning of a mouse smoothened cDNA and expression patterns of hedgehog signalling molecules during chondrogenesis and cartilage differentiation in clonal mouse EC cells, ATDC5
Cloning of a mouse smoothened cDNA and expression patterns of hedgehog signalling molecules during chondrogenesis and cartilage differentiation in clonal mouse EC cells, ATDC5
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DOI：
10.1006/bbrc.1997.6750
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发表时间：
1997-06-09
影响因子：
3.1
通讯作者：
Nakamura, T
中科院分区：
文献类型：
--
作者：
Akiyama, H;Shigeno, C;Nakamura, T
Hedgehog（hh）家族蛋白似乎在其信号传导途径中使用保守靶标，包括Patched（Ptc）、Smoothened（Smo）和Gli。虽然印度刺猬（Ihh）在软骨内骨形成中起着重要作用，但HH信号分子参与骨骼发生尚不清楚。我们克隆了小鼠（m）Smo cDNA，并研究了Ihh，Ptc，Smo和Gli mRNA在小鼠软骨形成EC细胞ATDC 5中的表达模式。推导的氨基酸序列由793个氨基酸组成，与大鼠（r）Smo和人（h）Smo的同源性分别为98%和93%。在ATDC 5细胞中，Ihh mRNA的表达与X型胶原mRNA、Smo、Ptc和Gli mRNA的表达一致，在整个软骨形成和随后的软骨分化过程中，除了在细胞凝聚阶段Ptc mRNA的瞬时减少外，其余均组成性表达。我们的数据表明，hh信号分子可能参与ATDC 5细胞的软骨形成和软骨分化。(C)北京：科学出版社.
Hedgehog (hh) family proteins appear to use the conserved targets in their signalling pathway including Patched (Ptc), Smoothened (Smo), and Gli. Although Indian hedgehog (Ihh) plays an important role in endochondral bone formation, the involvement of hh signalling molecules in skeletogenesis is unknown. We cloned a mouse (m) Smo cDNA and studied the expression patterns of Ihh, Ptc, Smo, and Gli mRNAs in mouse chondrogenic EC cells, ATDC5. The deduced amino acid sequence of mSmo consisted of 793 amino acids and was 98 and 93% homologous to the rat (r) Smo and human (h) Smo, respectively. In ATDC5 cells, the expression of Ihh mRNA paralleled that of type X collagen mRNA, Smo, Ptc, and Gli mRNAs were constitutively expressed throughout chondrogenesis and the subsequent cartilage differentiation processes except for the transient decrease in Ptc mRNA at the cellular condensation stage. Our data suggest that hh signalling molecules may be involved in chondrogenesis and cartilage differentiation in ATDC5 cells. (C) 1997 Academic Press.