Improved growth performance, food efficiency, and lysine availability in growing rats fed with lysine-biofortified rice.
Improved growth performance, food efficiency, and lysine availability in growing rats fed with lysine-biofortified rice.
复制标题
饲喂赖氨酸生物强化稻米的生长大鼠的生长性能、食物效率和赖氨酸可用性得到改善
DOI：
10.1038/s41598-017-01555-0
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发表时间：
2017-05-02
影响因子：
4.6
通讯作者：
Sun SS
中科院分区：
文献类型：
--
作者：
Yang QQ;Suen PK;Zhang CQ;Mak WS;Gu MH;Liu QQ;Sun SS
大米是蛋白质的绝佳来源，除了必需的氨基酸赖氨酸外，氨基酸具有足够的平衡。通过使用赖氨酸合成的结合增强和对其分解代谢的抑制，我们产生了两种转基因水稻系HFL1和HFL2（高游离赖氨酸），其中含有高浓度的游离赖氨酸。在这项研究中，进行了为期70天的大鼠喂养研究，以评估两种转基因线的营养价值与它们的野生型（WT）或补充不同浓度L-赖氨酸的WT水稻相比。结果表明，HFL组的动物性能，包括体重，食物摄入量和食物效率，比在WT组中更大。此外，与WT饮食相比，HFL饮食的蛋白质明显消化率，蛋白质效率比和赖氨酸的可用性增加。基于饮食中的L-赖氨酸浓度与动物性能之间的线性关系，它表明HFL组的生物指数与WT20组相似或更好，WT20组的生物指数与HFL饮食中存在的L-赖氨酸浓度相似。因此，赖氨酸生物植物的大米有助于提高生长大鼠的生长性能，粮食效率和赖氨酸的可用性。
Rice is an excellent source of protein, and has an adequate balance of amino acids with the exception of the essential amino acid lysine. By using a combined enhancement of lysine synthesis and suppression of its catabolism, we had produced two transgenic rice lines HFL1 and HFL2 (HighFreeLysine) containing high concentration of free lysine. In this study, a 70-day rat feeding study was conducted to assess the nutritional value of two transgenic lines as compared with either their wild type (WT) or the WT rice supplemented with different concentrations of L-lysine. The results revealed that animal performance, including body weight, food intake, and food efficiency, was greater in the HFL groups than in the WT group. Moreover, the HFL diets had increased protein apparent digestibility, protein efficiency ratio, and lysine availability than the WT diet. Based on the linear relationship between dietary L-lysine concentrations and animal performance, it indicated that the biological indexes of the HFL groups were similar or better than that of the WT20 group, which was supplemented with L-lysine concentrations similar to those present in the HFL diets. Therefore, lysine-biofortified rice contributed to improved growth performance, food efficiency, and lysine availability in growing rats.