Prevalence and Detection of Trichomonas vaginalis in HIV-Infected Pregnant Women.
Prevalence and Detection of Trichomonas vaginalis in HIV-Infected Pregnant Women.
复制标题
DOI：
10.1097/olq.0000000000000756
复制
发表时间：
2018-05
影响因子：
3.1
通讯作者：
Klausner JD
中科院分区：
文献类型：
--
作者：
Price CM;Peters RPH;Steyn J;Mudau M;Olivier D;De Vos L;Morikawa E;Kock MM;Medina-Marino A;Klausner JD
Trichomonas vaginalis is a STI associated with increased transmission of HIV and significant adverse birth outcomes; culture and PCR are commonly used in diagnosis. Consenting HIV-infected pregnant women were recruited from clinics in South Africa and screened for T. vaginalis using PCR. PCR-positive women provided an additional sample for culture. We compared T. vaginalis detection between PCR and culture, and investigated how PCR cycle threshold (Ct) values differ among culture results. A total of 359 women were enrolled and 76 (20%) tested T. vaginalis PCR-positive. Cultures were obtained from 61 of the PCR-positive women, and 38 (62%) were culture-positive. The median baseline Ct of the PCR-positive/culture-positive group was 22.6 vs. 38.0 among those PCR-positive/culture-negative (p<0.001). Culture positive cases had lower Ct values (higher DNA load); a Ct value < 30 predicted positivity with a sensitivity of 97% and specificity of 96%. Culture was positive in roughly half of PCR positive cases. The culture-negative cases had significantly higher Ct values, indicating a lower concentration of T. vaginalis DNA. A Ct value of 30 provides a reliable threshold for predicting culture positivity. The clinical significance of culture-negative infections detected by PCR is still unclear.
