Fibronectin 1 promotes migration and invasion of papillary thyroid cancer and predicts papillary thyroid cancer lymph node metastasis.
Fibronectin 1 promotes migration and invasion of papillary thyroid cancer and predicts papillary thyroid cancer lymph node metastasis.
复制标题
纤连蛋白1促进甲状腺乳头状癌的迁移和侵袭并预测甲状腺乳头状癌淋巴结转移
DOI：
10.2147/ott.s122009
复制
发表时间：
2017
影响因子：
4
通讯作者：
Zhan W
中科院分区：
文献类型：
--
作者：
Xia S;Wang C;Postma EL;Yang Y;Ni X;Zhan W
淋巴结转移（LNM）在甲状腺乳头状癌（PTC）中很常见，并且是复发的指标。LNM在PTC中的详细分子机制尚未得到很好的描述。本研究旨在探讨纤维连接蛋白1在PTC LNM中的作用及其临床意义。在PTC组织和细胞系中证实了纤维连接蛋白1的表达。进行相关性分析，并获得受试者工作特征曲线。使用集落形成测定和细胞计数试剂盒8进行纤连蛋白1对PTC细胞系增殖的影响。用流式细胞术测定进行细胞周期分析。通过transwell实验和伤口愈合实验评估细胞的迁移和侵袭能力。转移性PTC中纤连蛋白1过表达。纤维连接蛋白1过表达与PTC LNM呈正相关。受试者操作特征分析表明，纤连蛋白1诊断乳腺癌的准确性为81.1%，敏感性为80%，特异性为82%。纤维连接蛋白1的过表达促进PTC的增殖、迁移和侵袭。纤维连接蛋白1通过调节PTC细胞的增殖、迁移和侵袭能力在PTC转移中起关键作用，并且是预测PTC LNM的有价值的诊断生物标志物。
Lymph node metastasis (LNM) is common in papillary thyroid cancer (PTC), and is an indicator of recurrence. The detailed molecular mechanism of LNM in PTC has not been well described. This study aimed to investigate the role of fibronectin 1 in PTC LNM and its clinical relevance. The expression of fibronectin 1 was confirmed in PTC tissues and cell lines. A correlation analysis was conducted and a receiver-operating characteristic curve obtained. The effect of fibronectin 1 on the proliferation of PTC cell lines was performed using a colony-formation assay and Cell Counting Kit 8. Cell-cycle analysis was performed with a flow-cytometry assay. Migration and invasion ability were evaluated by transwell and wound-healing assays. Fibronectin 1 was overexpressed in metastasized PTC. Overexpressed fibronectin 1 was positively correlated with PTC LNM. Receiver-operating characteristic analysis showed that the diagnostic accuracy of fibronectin 1 was 81.1%, with sensitivity of 80% and specificity of 82%. Overexpression of fibronectin 1 promoted proliferation, migration, and invasion in PTC. Fibronectin 1 plays a critical role in PTC metastasis by modulating the proliferation, migration, and invasion ability of PTC cells, and it is a valuable diagnostic biomarker for predicting PTC LNM.