Oscillating focus of SopA associated with filamentous structure guides partitioning of F plasmid
Oscillating focus of SopA associated with filamentous structure guides partitioning of F plasmid
复制标题
DOI：
10.1111/j.1365-2958.2007.05728.x
复制
发表时间：
2007-06-01
影响因子：
3.6
通讯作者：
Niki, Hironori
中科院分区：
文献类型：
--
作者：
Hatano, Toshiyuki;Yamaichi, Yoshiharu;Niki, Hironori
F质粒通过sopABC基因主动分配给子细胞。为了阐明分配机制，我们同时分析了单个活细胞中质粒和SopA ATP酶的运动。SopA是一种假定的马达蛋白，其在类核边界附近密集组装，并形成与沿细胞长轴沿着较不密集的丝状分布相关的单个离散焦点。密集的SopA焦点在细胞两极之间振荡。质粒运动的方向随着SopA焦点位置的改变而改变。质粒运动的速度保持恒定，同时其朝向SopA焦点振荡移动。低密度丝状分布的SOPA持续整个SOPA振荡。在没有类核的细胞中也观察到与SopA的丝状分布相关的焦点。SopA丝可以像铁路轨道一样引导质粒的运动，并将其引导到细胞区。
The F plasmid is actively partitioned to daughter cells by the sopABC gene. To elucidate the partitioning mechanisms, we simultaneously analysed movements of the plasmid and the SopA ATPase in single living cells. SopA, which is a putative motor protein assembled densely near nucleoid borders and formed a single discrete focus associated with less dense filamentous distribution along the long axis of the cell. The dense SopA focus oscillates between cell poles. The direction of the plasmid motion switches as the SopA focus switches its position. The velocity of the plasmid motion stays constant while it oscillates moving towards the SopA focus. The low density filamentous distribution of SopA persisted throughout the SopA oscillation. The focus associated with filamentous distribution of SopA was also observed in a cell without nucleoid. The SopA filament may guide the movement of the plasmid as a railway track and lead it to cell quarters.