Modulating human connective tissue progenitor cell behavior on cellulose acetate scaffolds by surface microtextures.
Modulating human connective tissue progenitor cell behavior on cellulose acetate scaffolds by surface microtextures.
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通过表面微观纹理调节醋酸纤维素支架上的人类结缔组织祖细胞行为。
DOI：
10.1002/jbm.a.32160
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Roy,Shuvo
中科院分区：
文献类型：
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作者：
Kim,EunJung;Boehm,CynthiaA;Fleischman,AaronJ;Muschler,GeorgeF;Kostov,YordanV;Roy,Shuvo
采用软光刻技术制备具有表面微结构的醋酸纤维素(CA)支架，观察人骨髓来源的结缔组织前体细胞(CTPs)后代的生长特性。收集人CTP并在含有后微结构和光滑表面的CA支架上培养长达30天。光滑表面上的细胞在30天内无任何择优取向迁移。在微结构上，第9天，细胞倾向于向POST和其他细胞突起。到第30天，微结构上的细胞表面覆盖着细胞外基质。DNA定量显示，微纹理上的细胞数量大约是光滑表面上的三倍。第9天，碱性磷酸酶(AP)在光滑表面的表达略高，但到了第30天，AP mRNA在微结构上的表达有所增加。微结构中I型胶原的mRNA表达在30d时增加，而光滑表面的表达与之相似。30d时，相对于光滑表面，骨钙素在后微结构上的表达增加。#2008 Wiley期刊，Inc.生物材料研究杂志，2009
Soft lithography techniques are used to fabricate cellulose acetate (CA) scaffolds with surface microtextures to observe growth characteristics of the progeny of human marrow‐derived connective tissue progenitor cells (CTPs). Human CTPs were collected and cultured on CA scaffolds comprised postmicrotextures and smooth surfaces for up to 30 days. Cells on the smooth surfaces migrated without any preferred orientation for up to 30 days. On microtextures, cells tended to direct their processes toward posts and other cells on day 9. By day 30, cells on microtextures covered the surface with extracellular matrix. DNA quantification revealed approximately threefold more cells on microtextures than on the smooth surfaces. The alkaline phosphatase (AP) mRNA expression was slightly higher on smooth surfaces on day 9. However, by day 30, AP mRNA showed higher expression on microtextures. The mRNA expression of collagen type I was increased on microtextures by day 30, whereas smooth surfaces demonstrated similar expression. The osteocalcin mRNA expression was increased on postmicrotextures relative to smooth surfaces by day 30. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009