HAIRPIN LOOP STRUCTURE OF AFRICAN SWINE FEVER VIRUS-DNA
HAIRPIN LOOP STRUCTURE OF AFRICAN SWINE FEVER VIRUS-DNA
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DOI：
10.1093/nar/14.17.6835
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发表时间：
1986-09-11
影响因子：
14.9
通讯作者：
VINUELA, E
中科院分区：
文献类型：
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作者：
GONZALEZ, A;TALAVERA, A;VINUELA, E
非洲猪瘟病毒基因组的末端由 37 个核苷酸长的发夹环形成，该发夹环几乎完全由不完全配对的 A 和 T 残基组成。每个 DNA 末端的环以两种等摩尔形式存在，当以相反的极性进行比较时，它们是反向且互补的（触发器），就像痘病毒 DNA 的情况一样。非洲猪瘟病毒和痘苗病毒DNA的发夹环没有同源性，但两种DNA都有16个核苷酸长的序列，与发夹环接近，同源性约为80%。对非洲猪瘟病毒复制 DNA 的分析表明，头对头和尾对尾连接的分子可能是复制中间体。
The ends of African swine fever virus genome are formed by a 37 nucleotide-long hairpin loop composed, almost entirely, of incompletely paired A and T residues. The loops at each DNA end were present in two equimolar forms that, when compared in opposite polarities, were inverted and complementary (flip-flop), as in the case of poxvirus DNA. The hairpin loops of African swine fever and vaccinia virus DNAs had no homology, but both DNAs had a 16 nucleotide-long sequence, close to the hairpin loops, with an homology of about 80%. An analysis of African swine fever virus replicating DNA showed head-to-head and tail-to-tail-linked molecules that may be replicative intermediates.