Quantitation of dopaminergic neurons in 3-dimensional reaggregate tissue culture by computer-assisted image analysis.
Quantitation of dopaminergic neurons in 3-dimensional reaggregate tissue culture by computer-assisted image analysis.
复制标题
通过计算机辅助图像分析对 3 维重新聚集组织培养物中的多巴胺能神经元进行定量。
DOI：
10.1016/0165-0270(94)00110-3
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发表时间：
1995
影响因子：
3
通讯作者：
Heller,A
中科院分区：
文献类型：
--
作者：
Vidal,L;Heller,B;Won,L;Heller,A
Three-dimensional (3D) reaggregate tissue culture provides a means of quantitatively assessing neuronal cell survival under a variety of experimental conditions. A method is presented for estimation of, and comparison between, the total numbers of cells of a given neurochemical type within individual experimental flasks. The method involves cell counting from random reaggregate sections, determination of sectional volumes, and of 3D reaggregate volumes by a novel image-analysis computer system.
