Anthracnose: a new leaf disease on Radermachera sinica (China doll) in China
Anthracnose: a new leaf disease on Radermachera sinica (China doll) in China
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炭疽病：中国瓷娃娃的一种新叶部病害
DOI：
10.1094/pdis-01-22-0072-sc
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发表时间：
2022
期刊：
影响因子：
4.5
通讯作者：
Zifu He
中科院分区：
文献类型：
--
作者：
Lin Yu;Chuang Lyu;Y.F. Tang;Guobing Lan;Zhenggang Li;Xiaoman She;Zifu He
中国洋娃娃（Radermachera sinica）是一种世界性的万年青园艺作物。然而，很少有资料提供描述炭疽病的R。sinica。2018年，在R.中国广州的花园和商业温室中的sinica。病叶病斑中心变薄，呈灰白色，边缘呈深褐色至黑色，黑点凸起。27个单分生孢子分离物从症状叶片病变。根据形态特征和多位点系统发育分析，19个菌株被鉴定为暹罗炭疽菌，6个和2个菌株被鉴定为C. fructicola和C.岩溶作用。采用离体叶片法进行致病性测定。sinica植物，并发现C.暹罗虎在受伤的条件下比在未受伤的条件下更具攻击性，并在叶片上引起症状性坏死病变。然后，从接种叶片的病斑中重新分离出相同的病原体，以满足Koch的假设。而C. fructicola nor C. karstii引起的叶片可见病变。结果表明，它们可能是无症状内生菌或条件致病菌。sinica。据我们所知，本研究是首次报道炭疽。与炭疽病的发生有关。中国的Sinica。
Radermachera sinica (China doll) is a popular evergreen horticultural crop worldwide. However, little information has been provided describing the anthracnose disease of R. sinica. In 2018, symptoms suspected of leaf anthracnose were observed on R. sinica in gardens and commercial greenhouses in Guangzhou, China. Lesions on diseased leaves showed thinned and grayish white centers, dark-brown to black borders and raised black spots. Twenty-seven single-conidia isolates were obtained from symptomatic leaf lesions. Based on morphological characteristics and multi-locus phylogenetic analysis, nineteen isolates were identified as Colletotrichum siamense and six and two isolates were identified as C. fructicola and C. karstii, respectively. In vivo pathogenicity test was conducted on leaves of R. sinica plants and discovered that C. siamense was more aggressive under wounded conditions than under unwounded conditions and caused symptomatic necrotic lesions on the leaf. Afterwards, the same pathogen was re-isolated from lesions of inoculated leaves to fulfill Koch’s postulates. Whereas neither C. fructicola nor C. karstii caused visible lesions on leaves of R. sinica under wounded or unwounded conditions, indicating that they may be asymptomatic endophytes or opportunistic pathogens on R. sinica. To our knowledge, this study is the first report of Colletotrichum spp. associated with anthracnose disease on R. sinica in China.