Lab on a Chip Single-sided continuous optoelectrowetting ( SCOEW ) for droplet manipulation with light patterns †
Lab on a Chip Single-sided continuous optoelectrowetting ( SCOEW ) for droplet manipulation with light patterns †
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发表时间：
2010
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通讯作者：
Sung-Yong Park;Eric P. Y. Chiou
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作者：
Sung-Yong Park;Eric P. Y. Chiou
Chiou SCOEW用于利用光图案操纵液滴Haswell快速PCR扩增电介质润湿(EWOD)有望成为有效操纵具有电场控制表面张力的液滴的重要芯片实验室方法。在电润湿设备中操纵的液滴通常被夹在两个平行板之间，并由数字电极驱动。像素化电极的大小限制了可以操纵的最小液滴尺寸。在这里，我们报告了一种单面连续光电润湿(SCOEW)机制，该机制使光图案化电润湿调制能够在开放的、无特征的光导表面上连续操纵液滴。SCOEW通过利用动态和可重构的光学图案克服了物理像素化电极的尺寸限制，使体积从50毫升到250像素、超过5个数量级的液滴能够连续传输、分裂、合并和混合。这种单面开放结构提供了灵活的接口，用于与其他微流体组件集成，例如通过简单的管路实现样品储集器。SCOEW展示了体积变化小于1%的光触发、并行和体积可调的液滴喷射。独特的横向场驱动光电润湿机制还可以实现极低的光强驱动，通过将SCOEW芯片直接定位在笔记本电脑或便携式手机中使用的LCD屏幕上，可以实现液滴操纵。
Chiou SCOEW for droplet manipulation with light patterns Haswell Rapid PCR amplification Electrowetting-on-dielectric (EWOD) promises to be an important lab-on-a-chip approach for effectively manipulating droplets with electric field-controlled surface tension. Droplets manipulated in electrowetting-based devices are typically sandwiched between two parallel plates and actuated by digital electrodes. The size of pixilated electrodes limits the minimum droplet size that can be manipulated. Here, we report on a single-sided continuous optoelectrowetting (SCOEW) mechanism that enables light-patterned electrowetting modulation for continuous droplet manipulation on an open, featureless, and photoconductive surface. SCOEW overcomes the size limitation of physical pixilated electrodes by utilizing dynamic and reconfigurable optical patterns and enables the continuous transport, splitting, merging, and mixing of droplets with volumes ranging from 50 mL to 250 pL, over 5-orders of magnitude. This single-sided open configuration provides a flexible interface for integration with other microfluidic components, such as sample reservoirs through simple tubing. Light-triggered, parallel, and volume-tunable droplet injection with volume variation less than 1% has been demonstrated with SCOEW. The unique lateral field-driven optoelectrowetting mechanism also enables extremely low light intensity actuation, and droplet manipulation can be achieved by directly positioning the SCOEW chip on a LCD screen used in a laptop or portable cellular phone.