Abundant production of brain-derived neurotrophic factor by adult visceral epithelia -: Implications for paracrine and target-derived neurotrophic functions
Abundant production of brain-derived neurotrophic factor by adult visceral epithelia -: Implications for paracrine and target-derived neurotrophic functions
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DOI：
10.1016/s0002-9440(10)65221-2
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发表时间：
1999-10-01
影响因子：
6
通讯作者：
Renz, H
中科院分区：
文献类型：
--
作者：
Lommatzsch, M;Braun, A;Renz, H
脑源性神经营养因子（BDNF）在内脏感觉神经元的发育过程中起着至关重要的作用。然而，BDNF在成人脏器中的生理来源和功能尚不清楚。我们研究了BDNF在成年鼠脏器中的细胞来源和潜在作用。我们发现不同器官中BDNF蛋白的含量明显不同。令人惊讶的是，膀胱、肺和结肠中的BDNF水平高于大脑或皮肤中的BDNF水平。原位杂交实验显示，内脏上皮细胞、几种类型的平滑肌和肌丛神经元可以产生BDNF mRNA。表达BDNF的上皮细胞缺乏BDNF的高亲和力和低亲和力受体、trkB和p75（NTR）。相反，这两种受体都存在于周围神经系统的神经元上。对BDNF-/-小鼠的研究表明，上皮细胞和平滑肌细胞在缺乏BDNF的情况下发育正常。这些数据提供了证据，证明内脏上皮是成人内脏BDNF的主要来源，但不是目标。BDNF蛋白在某些内脏器官中的丰度表明，这种神经营养因子可能调节成人内脏感觉和运动神经元的功能。
Brain-derived neurotrophic factor (BDNF) plays a crucial role for the survival of visceral sensory neurons during development. However, the physiological sources and the function of BDNF in the adult viscera are poorly described. We have investigated the cellular sources and the potential role of BDNF in adult murine viscera. We found markedly different amounts of BDNF protein in different organs. Surprisingly, BDNF levels in the urinary bladder, lung, and colon were higher than those found in the brain or skin. In Situ hybridization experiments revealed that BDNF mRNA was made by visceral epithelial cells, several types of smooth muscle, and neurons of the myenteric plexus. Epithelia that expressed BDNF lacked both the high- and low-affinity receptors for BDNF, trkB and p75(NTR). In contrast, both receptors were present on neurons of the peripheral nervous system. Studies with BDNF-/-mice demonstrated that epithelial and smooth muscle cells developed normally in the absence of BDNF. These data provide evidence that visceral epithelia are a major source, but not a target, of BDNF in the adult viscera. The abundance of BDNF protein in certain internal organs suggests that this neurotrophin may regulate the function of adult visceral sensory and motor neurons.