Biotransformation of progesterone by Aspergillus nidulans VKPM F-1069 (wild type)
Biotransformation of progesterone by Aspergillus nidulans VKPM F-1069 (wild type)
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DOI：
10.1016/j.steroids.2019.05.013
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发表时间：
2019-09-01
期刊：
影响因子：
2.7
通讯作者：
Savinova, Tatiana S.
中科院分区：
文献类型：
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作者：
Savinova, Olga S.;Solyev, Pavel N.;Savinova, Tatiana S.
Biotechnological transformation of steroids using enzyme systems of microorganisms is often the only possible method to modify the molecule in the industrial production of steroid drugs. Filamentous fungus Aspergillus nidulans has been little studied as a steroid-transforming microorganism. We studied the ability of the A. nidulans VKPM F-1069 strain to transform progesterone (PG) for the first time. This strain converts PG into 3 main products: 11 alpha-hydroxy-PG, 11 alpha-acetoxy-PG and 6 beta,11 alpha-dihydroxy-PG. It has been established that in the first stage, the hydroxylation of PG occurs into Cl la position, then the formed 11 alpha-hydroxy-PG is modified into 11 alpha-acetoxy-PG and 6 beta,11 alpha-dihydroxy-PG. It was found that changes in the composition of the growth medium, aeration and the duration of the mycelium cultivation do not affect the qualitative composition of PG transformation products, but their ratios have changed. Under conditions of limited aeration, the direction of secondary modification of 11 alpha-hydroxy-PG is shifted towards the formation of 11 alpha-acetoxy-PG.
