Vortex-assisted dispersive liquid-liquid microextraction for the analysis of major Aspergillus and Penicillium mycotoxins in rice wine by liquid chromatography-tandem mass spectrometry
Vortex-assisted dispersive liquid-liquid microextraction for the analysis of major Aspergillus and Penicillium mycotoxins in rice wine by liquid chromatography-tandem mass spectrometry
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涡旋辅助分散液液微萃取液相色谱-串联质谱法分析黄酒中主要曲霉菌和青霉菌毒素
DOI：
10.1016/j.foodcont.2016.09.035
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发表时间：
2017-03-01
期刊：
影响因子：
6
通讯作者：
Zhou, Changyan
中科院分区：
文献类型：
--
作者：
Zhao, Zhiyong;Yang, Xianli;Zhou, Changyan
黄酒发酵过程中不可避免地会出现曲霉和青霉。黄酒安全性需要对这两种真菌产生的有毒次级代谢产物进行实际监测。本研究建立了一种快速、简便的涡旋辅助分散液液微萃取（VADLLME）技术，用于黄酒中主要曲霉和青霉毒素（黄曲霉毒素B1、黄曲霉毒素B2、黄曲霉毒素G1、黄曲霉毒素G2、赭曲霉毒素A、霉酚酸、杂色曲霉素和疣孢菌素）的提取和富集。采用响应面法结合Box-Behnken设计和期望函数法对影响VADLLME提取效率的关键参数（pH值、提取剂和分散剂的种类和体积）进行优化。通过液相色谱串联质谱法（LC-MS/MS）分析目标化合物。在最佳条件下，在0.5 ~ 100 μ g/L范围内，所有分析物的线性关系良好（R-2>0.99）。3个浓度水平的加标回收率在84.5%~ 111.2%之间，相对标准偏差（RSD）均小于20%。方法的检出限（LOD）和定量限（LOO）分别为0.05 ~ 0.5 μ g/L和0.1 ~ 1.0 μ g/L。该方法具有操作简单、成本低、有机溶剂用量少等优点。该方法已成功应用于黄酒样品的分析。发现黄酒中霉酚酸发生率较高，需持续监测。(C)2016爱思唯尔有限公司版权所有。
The presence of Aspergillus and Peniciltium species during the fermentation process of rice wine is inevitable. The toxic secondary metabolites produced by the two fungi need to be practically monitored for rice wine safety. In this study, we developed a fast and simple vortex-assisted dispersive liquid-liquid microextraction (VADLLME) for the extraction and preconcentration of major Aspergillus and Penicillium mycotoxins (aflatoxin Bl, aflatoxin B2, aflatoxin Gl, aflatoxin G2, ochratoxin A, mycophenolic acid, sterigmatocystin and verruculogen) in rice wine. The key parameters affecting the extraction efficiency of VADLLME procedure (pH value, type and volume of extraction and dispersive solvents) were optimized by response surface methodology (RSM) combined with Box-Behnken design (BBD) and desirability function. Target compounds were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). Under the optimum conditions, good linearity (R-2>0.99) was achieved for all the analytes in the concentration range of 0.5-100 mu g/L. The recoveries for spiked samples at three concentration levels were between 84.5% and 111.2%, with the relative standard deviations (RSD) lower than 20%. The limits of detection (LOD) and quantification (LOO ranged from 0.05 to 0.5 mu g/L and from 0.1 to 1.0 mu g/L, respectively. Simplicity in operation, low cost and low usage of organic solvents were the major advantages of the developed method. The method was successfully applied to the analysis of rice wine samples. It was found that mycophenolic acid had high incidence rate in rice wine, which needed to be continuously monitored. (C) 2016 Elsevier Ltd. All rights reserved.