The timing for neuronal maturation in the adult hippocampus is modulated by local network activity.
The timing for neuronal maturation in the adult hippocampus is modulated by local network activity.
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DOI：
10.1523/jneurosci.1380-11.2011
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发表时间：
2011-05-25
期刊：
影响因子：
--
通讯作者：
Schinder AF
中科院分区：
文献类型：
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作者：
Piatti VC;Davies-Sala MG;Espósito MS;Mongiat LA;Trinchero MF;Schinder AF
成年海马体不断产生新的未成熟神经元群，其兴奋性和可塑性增强。每个队列中这些独特特性的表达窗口由获得成熟神经元表型所需的时间决定。在这里，我们表明局部网络活动调节沿海马间隔颞轴的成年神经元的成熟率。结合共聚焦显微镜和膜片钳记录来评估逆转录病毒标记的神经元随时间的标记表达、形态发育和功能特性。与颞区相比，间隔齿状回表现出更高水平的基础网络活动和更快的新生神经元成熟率。自愿锻炼仅增强了颞区的网络活动，进而加速了神经元的发育。最后，当神经元内在电活动因细胞自主过度表达 Kir2.1（一种内向整流钾通道）而降低时，在高度活跃的环境中发育的神经元表现出成熟延迟。我们的研究结果揭示了一种新型的活动依赖性可塑性，作用于神经元成熟的时间和沿成年海马纵轴新生成的神经元的功能整合。
The adult hippocampus continuously generates new cohorts of immature neurons with increased excitability and plasticity. The window for the expression of those unique properties in each cohort is determined by the time required to acquire a mature neuronal phenotype. Here, we show that local network activity regulates the rate of maturation of adult-born neurons along the septotemporal axis of the hippocampus. Confocal microscopy and patch-clamp recordings were combined to assess marker expression, morphological development, and functional properties in retrovirally labeled neurons over time. The septal dentate gyrus displayed higher levels of basal network activity and faster rates of newborn neuron maturation than the temporal region. Voluntary exercise enhanced network activity only in the temporal region and, in turn, accelerated neuronal development. Finally, neurons developing within a highly active environment exhibited a delayed maturation when their intrinsic electrical activity was reduced by the cell-autonomous overexpression of Kir2.1, an inward-rectifying potassium channel. Our findings reveal a novel type of activity-dependent plasticity acting on the timing of neuronal maturation and functional integration of newly generated neurons along the longitudinal axis of the adult hippocampus.