Direct visualization of a molecular handshake that governs kin recognition and tissue formation in myxobacteria
Direct visualization of a molecular handshake that governs kin recognition and tissue formation in myxobacteria
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DOI：
10.1038/s41467-019-11108-w
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发表时间：
2019-07-12
影响因子：
16.6
通讯作者：
Wall, Daniel
中科院分区：
文献类型：
--
作者：
Cao, Pengbo;Wall, Daniel
许多生物体通过亲属识别来调节其社会生活，但其潜在机制却知之甚少。在这里，我们使用一种社会细菌，黄粘球菌，在分子水平上研究亲属识别。通过直接观察细胞表面受体 TraA，我们展示了这些粘细菌如何识别亲缘关系并过渡到多细胞性。 TraA 是细胞表面的液体，来自并列细胞的 TraA 之间的同型相互作用会触发受体结合，代表“分子握手”。 TraA 内的多态性控制着社会识别，使得受体仅聚集在具有相容等位基因的个体之间。 TraA 簇类似于真核间隙连接，指导细胞物质的稳健交换，使异质群体向体内平衡过渡。这项工作为微生物如何利用流体外膜受体识别亲缘细胞并将其组装成类似于组织的协作多细胞群落提供了概念框架。
Many organisms regulate their social life through kin recognition, but the underlying mechanisms are poorly understood. Here, we use a social bacterium, Myxococcus xanthus, to investigate kin recognition at the molecular level. By direct visualization of a cell surface receptor, TraA, we show how these myxobacteria identify kin and transition towards multicellularity. TraA is fluid on the cell surface, and homotypic interactions between TraA from juxtaposed cells trigger the receptors to coalesce, representing a 'molecular handshake'. Polymorphisms within TraA govern social recognition such that receptors cluster only between individuals bearing compatible alleles. TraA clusters, which resemble eukaryotic gap junctions, direct the robust exchange of cellular goods that allows heterogeneous populations to transition towards homeostasis. This work provides a conceptual framework for how microbes use a fluid outer membrane receptor to recognize and assemble kin cells into a cooperative multicellular community that resembles a tissue.