The effect of estradiol on hormonally stimulable adenylyl cyclase activity and on progesterone production in normal and regressing corpora lutea from control and human chorionic gonadotropin-treated pseudopregnant rabbits.
The effect of estradiol on hormonally stimulable adenylyl cyclase activity and on progesterone production in normal and regressing corpora lutea from control and human chorionic gonadotropin-treated pseudopregnant rabbits.
复制标题
雌二醇对对照和人绒毛膜促性腺激素处理的假孕兔的正常和退化黄体中激素刺激的腺苷酸环化酶活性和黄体酮产生的影响。
作者：
S. Day;L. Birnbaumer
研究了对未接受其他处理（对照组）或接受两种雌二醇（E - 1.5或E - 15）处理之一的9日龄假孕兔给予溶黄体剂量的人绒毛膜促性腺激素（100 IU，静脉注射）的效果。E - 1.5和E - 15分别为每12小时1.5μg雌二醇（E）和每12小时15μg E；这些处理从第8天22：00开始在芝麻油中皮下注射，并持续到第11天22：00。从第9 - 12天每天对动物采血，并在第12天10：00 - 11：00之间处死动物，此时测定血清和黄体（CL）孕酮含量、重量以及由促黄体生成素（10μg/ml）和异丙肾上腺素（Iso；10⁻⁴m）刺激的腺苷酸环化酶活性。我们发现：1）低剂量（E - 1.5）和高剂量（E - 15）的E处理均不能阻止人绒毛膜促性腺激素诱导的功能性黄体溶解（血清孕酮下降）；2）E - 15而非E - 1.5可阻止人绒毛膜促性腺激素诱导的结构性黄体溶解（CL重量减轻）；3）E - 15而非E - 1.5导致在人绒毛膜促性腺激素作用后CL孕酮水平难以区分……
The effect of administering a luteolytic dose of hCG (100 IU, iv) to 9-day pseudopregnant rabbits that received either no other treatment (controls) or one of two estradiol (E-1.5 or E-15) treatments was studied. E-1.5 and E-15 consisted of 1.5 μg estradiol (E) every 12 h and 15 μg E every 12 h, respectively; these treatments were administered sc in sesame seed oil starting at 2200 h on day 8 and continued through 2200 h on day 11. Animals were bled daily from days 9–12 and sacrificed between 1000–1100 h on day 12, at which time serum and corpora lutea (CL) progesterone content, weight, and adenylyl cyclase activities stimulated by LH (10 μg/ml) and isoproterenol (Iso; 10−4m) were determined. We found that 1) neither low (E-1.5) nor high (E-15) E treatment prevented functional luteolysis (drop in serum progesterone) induced by hCG; 2) E-15 but not E-1.5 prevented structural luteolysis (CL weight loss) induced by hCG; 3) E-15 but not E-1.5 resulted in CL progesterone levels that, after hCG, were indistingu...