Characterization of influenza virus PR8 strain cultured in embryonated eggs by cryo-electron tomography
Characterization of influenza virus PR8 strain cultured in embryonated eggs by cryo-electron tomography
复制标题
通过冷冻电子断层扫描表征在胚蛋中培养的流感病毒 PR8 株
DOI：
10.1016/j.bbrc.2019.05.161
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发表时间：
2019-08-13
影响因子：
3.1
通讯作者：
Yin, Changcheng
中科院分区：
文献类型：
--
作者：
Chen, Qiang;Huang, Xinrui;Yin, Changcheng
甲型流感病毒作为季节性流行病和周期性世界性大流行病的病原体，在全球范围内造成巨大的死亡损失。在鸡蛋中培养的PR 8株被广泛用于科学研究和流感疫苗的生产。本文利用冷冻电镜技术（CET）分析了流感病毒PR 8株在不同pH条件下的形态结构特征。我们发现大量的缺陷病毒体在含胚卵中繁殖。通过比较具有/不具有基质层的病毒体，揭示了在流感病毒生命周期期间，基质层在病毒体的结构完整性和RNP包封中起着至关重要的作用。我们还利用含有血凝素受体的脂质体来模拟膜融合过程。在膜融合过程中HA的几个潜在的中间体观察在酸性pH值。我们的观察提供深入了解流感病毒的结构和功能。(C)2019爱思唯尔公司All rights reserved.
Influenza A viruses, as causative agents of seasonal epidemics and periodic worldwide pandemics, cause enormous mortality loss globally. The PR8 strain cultured in chicken eggs is widely used for scientific research and the production of influenza vaccines. Here, based on Cryo-electron Tomography (CET), we analyzed the morphological and structural characteristics of the influenza virus PR8 strain at different pHs. We found that a large number of defective virions were propagated in embryonated eggs. By comparing virions with/without the matrix layer, it was revealed that the matrix layer played an essential role in the structural integrity of virions and RNPs encapsulation during the influenza virus life cycle. We also utilized hemagglutinin receptor-containing liposomes to mimic the membrane fusion process. Several potential intermediates of HA during membrane fusion were observed at acidic pH. Our observations afford insight into the architecture and function of influenza virus. (C) 2019 Elsevier Inc. All rights reserved.