Short communication: Genome-wide identification of new reference genes for reverse-transcription quantitative PCR in Streptococcus thermophilus based on RNA-sequencing analysis
Short communication: Genome-wide identification of new reference genes for reverse-transcription quantitative PCR in Streptococcus thermophilus based on RNA-sequencing analysis
复制标题
基于RNA测序分析的嗜热链球菌逆转录定量PCR新参考基因的全基因组鉴定
DOI：
10.3168/jds.2020-18672
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发表时间：
2020-11-01
影响因子：
3.5
通讯作者：
Ai, Lian-Zhong
中科院分区：
文献类型：
--
作者：
Xiong, Zhi-Qiang;Fan, Yi-Zhou;Ai, Lian-Zhong
嗜热链球菌是一种重要的工业乳酸菌，广泛应用于酸奶、奶酪等发酵乳制品的生产。基于基因表达的分析(例如，反转录定量实时聚合酶链式反应)的准确性在很大程度上依赖于可靠的参考基因(RG)的选择，这为正确解释表达数据提供了基础。然而，许多传统的RG在不同的系统中并不稳定表达。在这里，我们使用RNA测序在基因组水平上系统地研究基因表达的变异，并在嗜热链霉菌中鉴定出更稳定的RG。总共确定了21个具有变异系数值的候选RC
Streptococcus thermophilus, one of the most important industrial lactic acid bacteria, is widely used for the production of fermented dairy products such as yogurt and cheese. The accuracy of gene expression-based analyses (e.g., reverse-transcription quantitative real-time PCR) relies heavily on the selection of reliable reference genes (RG), which provides the basis for correctly interpreting expression data. However, many traditional RG are not stably expressed in different systems. Here we used RNA-sequencing to systematically investigate gene expression variation at the genome scale and identify more stable RG in S. thermophilus. In total, 21 putative candidate RC were identified with variation coefficient values