Surveillance of Acute Respiratory Infections Using Community-Submitted Symptoms and Specimens for Molecular Diagnostic Testing.
Surveillance of Acute Respiratory Infections Using Community-Submitted Symptoms and Specimens for Molecular Diagnostic Testing.
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DOI：
10.1371/currents.outbreaks.0371243baa7f3810ba1279e30b96d3b6
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发表时间：
2015-05-27
期刊：
影响因子：
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通讯作者：
Chunara, Rumi
中科院分区：
文献类型：
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作者：
Goff, Jennifer;Rowe, Aaron;Chunara, Rumi
急性呼吸道感染监测参与式系统提供了社区中流行感染的实时信息，但迄今为止仅局限于自我报告的综合征信息，缺乏将症状报告与感染类型联系起来的方法。我们开发了GoViral平台，以评估一群非专业志愿者是否能够在线提供自我报告的症状，并比较样本类型，以便为呼吸道感染监测提供足够质量的自我收集的诊断信息。招募志愿者，给他们一个工具包（收集材料和定制的说明），指示他们每周报告他们的症状，当出现感冒或流感样症状时，要求收集标本（唾液和鼻拭子）。我们比较了呼吸道病毒检测的敏感性（通过聚合酶链反应）和采集的容易程度。研究人员对参与者进行了调查，以确定他们在生病时参加活动的接受程度，以及接受有关导致他们感染的病原体类型和在他们附近传播的病原体类型的信息的接受程度。在2013年12月1日至2014年3月1日期间，295名参与者参加了这项研究，并收到了一个工具包。在报告症状的人中，有一半（71人）收集并送去标本进行分析。参与者在症状开始后平均2.30天（95 CI: 1.65至2.96）提交试剂盒。我们发现鼻腔和唾液样本对多种病原体具有良好的一致性，几乎没有差异。个人报告说，唾液收集是最容易的，并报告说，收到关于他们和他们附近的病原体的信息是有价值的，可以塑造公共卫生行为。社区提交的标本可用于检测急性呼吸道感染，个体表现出参与的接受性和对其附近呼吸道病原体的实时图像感兴趣。
Participatory systems for surveillance of acute respiratory infection give real-time information about infections circulating in the community, yet to-date are limited to self-reported syndromic information only and lacking methods of linking symptom reports to infection types. We developed the GoViral platform to evaluatewhether a cohort of lay volunteers could, andwould find ituseful to, contribute self-reported symptoms onlineand to compare specimen types for self-collected diagnostic information of sufficient quality for respiratory infection surveillance. Volunteers were recruited, given a kit (collection materials and customized instructions), instructed to report their symptoms weekly, and when sick with cold or flu-like symptoms, requested to collect specimens (saliva and nasal swab). We compared specimen types for respiratory virus detection sensitivity (via polymerase-chain-reaction) and ease of collection. Participants were surveyed to determine receptivity to participating when sick, to receiving information on the type of pathogen causing their infection and types circulating near them. Between December 1 2013 and March 1 2014, 295 participants enrolled in the study and received a kit. Of those who reported symptoms, half (71) collected and sent specimens for analysis. Participants submitted kits on average 2.30 days (95 CI: 1.65 to 2.96) after symptoms began. We found good concordance between nasal and saliva specimens for multiple pathogens, with few discrepancies.Individuals report that saliva collection is easiest and report that receiving information about what pathogen they, and those near them, have is valued and can shape public health behaviors. Community-submitted specimens can be used for the detection of acute respiratory infection with individuals showing receptivity for participating and interest ina real-time picture of respiratory pathogens near them.