Decoding the transcriptome of pre-granulosa cells during the formation of primordial follicles in the mouse
Decoding the transcriptome of pre-granulosa cells during the formation of primordial follicles in the mouse
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DOI：
10.1093/biolre/ioab065
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发表时间：
2021-04-13
影响因子：
3.6
通讯作者：
Saga, Yumiko
中科院分区：
文献类型：
--
作者：
Fukuda, Kurumi;Muraoka, Masafumi;Saga, Yumiko
原始卵泡是哺乳动物卵巢中有限的卵子储存库，由单个卵母细胞及其支持的体细胞组成，称为颗粒细胞。尽管它们的形成可能需要卵母细胞和颗粒前细胞(颗粒细胞的前体)之间的相互作用，但对其潜在的机制知之甚少。我们通过解码原始卵泡形成过程中颗粒前细胞的转录组来解决这个问题。我们发现，随着原始卵泡的形成，显著的基因表达变化，包括细胞外基质、细胞黏附和几个信号通路，都会发生。重要的是，在生殖细胞特异性基因Nanos3和fig1a的突变卵巢中，Lgr5-EGFP阳性的颗粒前细胞向FOXL2阳性的颗粒细胞的分化延迟，同时伴随着突变的颗粒前细胞中基因表达的扰动。这些结果表明，卵母细胞的正常发育是颗粒前细胞分化的必要条件。我们的数据为了解参与原始卵泡形成的基因调控网络提供了有价值的资源。摘要小鼠颗粒前细胞中的基因表达谱与原始卵泡的形成相一致，而卵母细胞的发育有助于这一过程。
Primordial follicles, a finite reservoir of eggs in mammalian ovaries, are composed of a single oocyte and its supporting somatic cells, termed granulosa cells. Although their formation may require reciprocal interplay between oocytes and pre-granulosa cells, precursors of granulosa cells, little is known about the underlying mechanisms. We addressed this issue by decoding the transcriptome of pre-granulosa cells during the formation of primordial follicles. We found that marked gene expression changes, including extracellular matrix, cell adhesion, and several signaling pathways, occur along with primordial follicle formation. Importantly, differentiation of Lgr5-EGFP-positive pre-granulosa cells to FOXL2-positive granulosa cells was delayed in mutant ovaries of the germ cell-specific genes Nanos3 and Figla, accompanied by perturbed gene expression in mutant pre-granulosa cells. These results suggest that proper development of oocytes is required for the differentiation of pre-granulosa cells. Our data provide a valuable resource for understanding the gene regulatory networks involved in the formation of primordial follicles.Summary sentenceGene expression profile in mouse pre-granulosa cells alters coinciding with primordial follicle formation and oocyte development contributes to this process.[GRAPHICS].