Identification and characterization of nine PAT1 branch genes in poplar
Identification and characterization of nine PAT1 branch genes in poplar
复制标题
杨树9个PAT1分支基因的鉴定与表征
DOI：
10.1007/s10725-016-0212-9
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发表时间：
2017-03-01
影响因子：
4.2
通讯作者：
Xu, Meng
中科院分区：
文献类型：
--
作者：
Cai, Heng;Xuan, Lei;Xu, Meng
光敏色素A信号转导1（Phytochrome A signal transduction 1，PAT 1）亚家族是GRAS家族的一个分支，在光敏色素信号转导和转录调控中发挥着重要作用，参与植物的发育过程。本研究利用白杨基因组芯片技术，初步研究了PAT 1分支基因在杨树不定根发生中的作用。在这项研究中，我们分离和鉴定了9个假定的PAT 1分支基因从杂交白杨。多重序列比对显示，这些蛋白质含有保守的C-末端GRAS结构域和高度可变的N-末端，进一步的系统发育分析证实这些基因属于PAT 1亚家族。9个PAT 1分支基因的时空表达谱显示，在不定根发育过程中有较高的表达水平。最后，亚细胞定位显示大多数PePAT 1定位于细胞核，而PePAT1.1定位于细胞质。因此，我们的研究结果表明，9个PAT 1分支基因可能与杨树不定根的发育有关，为进一步研究PAT 1亚家族在杨树中的功能奠定了基础。
The phytochrome A signal transduction 1 (PAT1) subfamily is a widely known branch of the GRAS family that has diverse regulatory roles in phytochrome signal transduction and transcriptional regulation, which is involved in the developmental processes of plants. Our previous study, using GeneChip Poplar Genome Arrays, indicated that PAT1 branch genes are possibly involved in adventitious root development in Populus. In this study, we isolated and characterized nine putative PAT1 branch genes from a hybrid poplar. A multiple sequence alignment revealed that the proteins contained substantially conserved C-terminal GRAS domains and highly variable N-terminals, and a further phylogenetic analysis confirmed that these genes belong to the PAT1 subfamily. Temporal and spatial expression profiles of the nine PAT1 branch genes showed high expression levels during adventitious root development. Finally, the subcellular localization showed that most of PePAT1s were localized to the nucleus, whereas PePAT1.1 was localized to the cytoplasm. Thus, our results indicated that nine PAT1 branch genes might be associated with adventitious root development, and they provided the basis for future research on the functions of the PAT1 subfamily in Populus.