Osteocytic osteolysis observed in rats to which parathyroid hormone was continuously administered
Osteocytic osteolysis observed in rats to which parathyroid hormone was continuously administered
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DOI：
10.1007/s00774-004-0519-x
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发表时间：
2004-11-01
影响因子：
3.3
通讯作者：
Ozawa, H
中科院分区：
文献类型：
--
作者：
Tazawa, K;Hoshi, K;Ozawa, H
为了证明体内骨细胞性骨质溶解，通过输液泵连续向8月龄大鼠给予人甲状旁腺激素（hPTH（1-34），749 ng/h）或相同体积的溶剂4周，然后从形态计量学、组织学和组织化学角度分析胫骨皮质骨中骨细胞的结构变化。根据接触显微放射照相术（CMR）观察，与对照组相比，PTH组的骨细胞陷窝有扩大的趋势，而PTH组的平均陷窝面积为137.0 mum（2），对照组为93.9 mum（2），表明存在骨细胞性骨溶解的证据。在PTH组的一些骨细胞中观察到酸性磷酸酶酶组织化学定位;因此，溶酶体系统可能参与溶骨机制。在苏木精-伊红或甲苯胺蓝染色的组织学样本上，对照组的腔隙被苏木精阳性和甲苯胺蓝异染的基质狭窄区域包围，而在PTH组腔隙周围观察到苏木精阳性的带状区域。这些发现表明，在骨细胞性骨质溶解后，再生骨基质可能被添加到具有扩大的骨陷窝的骨细胞壁中。
In order to prove osteocytic osteolysis in vivo, human parathyroid hormone (hPTH (1-34), 749 ng/h), or only solvent of the same volume, was continuously administered to 8-month-old rats by an infusion pump for 4 weeks, and then structural changes in osteocytes in the cortical bones of the tibiae were analyzed morphometrically, histologically, and histochemically. Based on contact microradiography (CMR) observations, the osteocyte lacunae in the PTH group tended to be enlarged, compared with those of the control, while the average lacuna area was 137.0mum(2) in the PTH group versus 93.9 mum(2) in the control, suggesting evidence of osteocytic osteolysis. Acid phosphatase enzyme histochemical localization was observed in some osteocytes in the PTH group; therefore, lysosome systems may participate in the osteolytic mechanisms. On histological samples stained with hematoxylin-eosin or toluidine blue, the lacunae of the controls were surrounded by narrow areas of matrices both positive for hematoxylin and metachromatic for toluidine blue, while belt-like areas positive for hematoxylin were observed around the PTH-group lacunae. These findings suggested that, after osteocytic osteolysis, regenerated bone matrices may be added to the walls of osteocytes that possess enlarged lacunae.