Differential expression of corticotropin-releasing hormone in developing mouse embryos and adult brain.
Differential expression of corticotropin-releasing hormone in developing mouse embryos and adult brain.
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DOI：
10.1210/endo.134.6.8194481
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发表时间：
1994-06
期刊：
影响因子：
4.8
通讯作者：
C E Keegan;J P Herman;I. Karolyi;K S O'Shea;S A Camper;A. Seasholtz
中科院分区：
文献类型：
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作者：
C E Keegan;J P Herman;I. Karolyi;K S O'Shea;S A Camper;A. Seasholtz
通过原位杂交组织化学在成年小鼠大脑的许多区域中检测到 CRH mRNA，其中最显着的是下丘脑的室旁核 (PVN)、下橄榄核和巴林顿核。肾上腺切除术后，稳态 CRH mRNA 水平增加了 1.7 倍，特别是在 PVN 中，这与糖皮质激素对大鼠 PVN 中 CRH 表达的负调节的报道一致。对胎儿和新生小鼠大脑中 CRH 表达的个体遗传学分析表明，在胚胎第 13.5 天，PVN、巴灵顿核、橄榄复合体和杏仁原基中存在 CRH mRNA。相比之下，直到出生后，皮质中的 CRH mRNA 才被检测到。 CRH 表达在个体发育中也表现出差异调节。 CRH mRNA 在每个脑区明显不同的发育时期达到成人水平，并且 CRH 表达在出生前和应激低反应期的 PVN 中特异性降低。高水平的 CRH mRNA 短暂存在于发育中的肺和腹腔神经节中。在糖皮质激素诱导的肺成熟期间胎儿肺中和交感神经系统发育期间腹腔神经节中CRH表达的新发现表明，CRH除了在激活应激反应中的作用之外，可能还具有一些重要的发育功能。
CRH mRNA was detected by in situ hybridization histochemistry in numerous regions of the adult mouse brain, including most prominently the paraventricular nucleus (PVN) of the hypothalamus, the inferior olivary nucleus, and Barrington's nucleus. After adrenalectomy, steady state CRH mRNA levels increased 1.7-fold, specifically in the PVN, consistent with reports of negative glucocorticoid regulation of CRH expression in the rat PVN. Ontogenetic analysis of CRH expression in fetal and neonatal mouse brain demonstrated CRH mRNA in PVN, Barrington's nucleus, olivary complex, and amygdaloid primordia on embryonic day 13.5. In contrast, CRH mRNA was not detectable in the cortex until after birth. CRH expression also exhibited differential regulation in ontogeny. CRH mRNA reached adult levels at markedly different times of development in each brain region, and CRH expression was reduced specifically in the PVN just before birth and the stress hyporesponsive period. High levels of CRH mRNA were present transiently in the developing lung and celiac ganglion. The novel findings of CRH expression in fetal lung during the period of glucocorticoid-induced lung maturation and in celiac ganglion during development of the sympathetic nervous system indicate that CRH may have some important developmental functions in addition to its role in activation of the stress response.