Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer
Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer
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发表时间：
2017-10
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通讯作者：
A. Bagheri;H. K. Khorram Khorshid;S. Mowla;H. Mohebbi;A. Mohammadian;M. Yaseri;M. Solaymani-Dodaran;M. Sherafatian;M. Tavallaie
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作者：
A. Bagheri;H. K. Khorram Khorshid;S. Mowla;H. Mohebbi;A. Mohammadian;M. Yaseri;M. Solaymani-Dodaran;M. Sherafatian;M. Tavallaie
背景资料：非小细胞肺癌（NSCLC）的早期诊断是一个艰巨的挑战，由于缺乏特异性的非侵入性标志物。microRNAs（miRNAs）在NSCLC的发生和发展中起重要作用。检测miRNA表达水平可能为NSCLC的诊断提供一种潜在的方法。我们的目标是检测NSCLC患者和无癌受试者的组织和痰液中miR-223、miR-212、miR-192、miR-3074、SNORD33和SNORD37的表达水平，用于NSCLC的分子诊断。研究方法：采用实时荧光定量RT-PCR方法检测17例NSCLC患者和17例对照者的组织和痰液中miR-223、miR-212、miR-192、miR-3074、SNORD33和SNORD37的相对表达。结果：与对照组相比，NSCLC患者组织中miR-3074表达上调。与对照组相比，患者痰液样本中miR-223上调，miR-212和SNORD37下调。miR-223定量检测NSCLC的灵敏度为82%，特异性为95%，ROC曲线下面积为0.90。结论：miR-223可以清楚地区分癌症患者和非癌症受试者，我们的研究结果表明，miR-223可能是一种诊断有用的生物标志物。痰液样本中改变的miRNA表达的测量表明了检测肺癌的潜在非侵入性方法。
Background: Diagnosis of Non-small Cell Lung Cancer (NSCLC) at an early stage is a daunting challenge due to the deficiency of specific noninvasive markers. MicroRNAs (miRNAs) play important roles in the initiation and progression of NSCLC. Measuring miRNA expression levels could provide a potential approach for the diagnosis of NSCLC. Our goals were to examine miR-223, miR-212, miR-192, miR-3074, SNORD33 and SNORD37 expression levels in tissue and sputum of NSCLC patients and cancer free subjects for molecular diagnosis of NSCLC. Methods: Relative expressions of miR-223, miR-212, miR-192, miR-3074, SNORD33 and SNORD37 were examined with quantitative real-time RT-PCR assay in tissue and sputum obtained from 17 NSCLC patients and 17 controls. Results: miR-3074 was upregulated in tissue samples of NSCLC patients compared with control group. miR-223 was upregulated, miR-212 and SNORD37 were downergulated in sputum samples of patients compared with controls. miR-223 quantification produced 82% sensitivity and 95% specificity with areas under the ROC curve at 0.90 in detection of NSCLC. Conclusion: miR-223 clearly discriminated cancer patients from cancer-free subjects and our results suggest that miR-223 could be a diagnostic useful biomarker. The measurement of altered miRNA expression in sputum samples manifested the potential noninvasive approach for detection of lung cancer.