Bladder function in female rats: effects of aging and pregnancy.
Bladder function in female rats: effects of aging and pregnancy.
复制标题
雌性大鼠的膀胱功能：衰老和妊娠的影响。
DOI：
10.1016/s0031-9384(99)00175-4
复制
发表时间：
1999
影响因子：
2.9
通讯作者：
Marson,L
中科院分区：
文献类型：
--
作者：
Wilfehrt,HM;Carson3rd,CC;Marson,L
在三组雌性大鼠中检查了体内麻醉膀胱测压图和体外膀胱组织条反应：年轻处女（3个月），老年处女（8个月）和退休饲养员（8-9个月）。观察到显著的年龄相关的体内变化，包括更大的静息压，但更小的排尿量，排尿持续时间和排尿间隔在老年与年轻的处女大鼠。在体外反应中存在显著的年龄相关变化。与老年动物相比，在年轻动物中观察到高K+修饰的Krebs（80 mM）去极化的峰值和稳态收缩更大。此外，年轻处女表现出更大的敏感性，但较小的最大，正常收缩乙酰胆碱（ACh）比老年处女。在年轻与年老的未交配大鼠中检测到对腺苷-5 ′-三磷酸（ATP）的反应减弱。研究人员比较了退休的饲养员和年龄匹配的对照组（年长的处女鼠）之间与怀孕相关的变化。在体内排尿量更大的退休育种比在老年处女。较小的在体外稳态收缩高K+-修改Krebs去极化和较小的归一化收缩，以最大浓度的乙酰胆碱中观察到的退休育种比在老处女。与年长的处女相比，退休的繁殖者对去甲肾上腺素的放松反应减弱。ATP产生更大的剂量依赖性反应和更大的最大收缩在退休的育种相比，老处女。总之，与年龄相关的变化甚至在衰老开始之前就存在，多胎妊娠改变了膀胱功能。
In vivo anesthetized cystometrograms and in vitro bladder tissue strip responses were examined in three groups of female rats: young virgins (3 month), older virgins (8 month), and retired breeders (8–9 month). Significant age-related in vivo changes were observed including greater resting pressures, but smaller voided volumes, void durations and void-to-void intervals in older versus young virgin rats. There were significant age-related changes in the in vitro responses. Greater peak and steady state contractions to high K+-modified Krebs (80 mM) depolarization were observed in young animals compared to older animals. Plus, young virgins exhibited greater sensitivity but smaller maximal, normalized contractions to acetylcholine (ACh) than older virgins. Diminished responses to adenosine-5′-triphosphate (ATP) were detected in young versus older virgin rats. Pregnancy-related changes were compared between retired breeders and their age-matched controls, older virgin rats. In vivo voided volumes were greater in the retired breeders than in the older virgins. Smaller in vitro steady state contractions to high K+-modified Krebs depolarization and smaller normalized contractions to maximal concentrations of ACh were observed in the retired breeders than in the older virgins. Retired breeders exhibited diminished relaxation responses to norepinephrine compared to older virgins. ATP produced greater dose-dependent responses and greater maximal contractions in the retired breeders compared to the older virgins. In conclusion, age-related changes were present even prior to the onset of senescence, and multiple pregnancies altered bladder function.