RECEPTOR-MEDIATED INTERNALIZATION OF FLUORESCENT CHEMOTACTIC PEPTIDE BY HUMAN-NEUTROPHILS
RECEPTOR-MEDIATED INTERNALIZATION OF FLUORESCENT CHEMOTACTIC PEPTIDE BY HUMAN-NEUTROPHILS
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DOI：
10.1126/science.472759
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发表时间：
1979-01-01
期刊：
影响因子：
56.9
通讯作者：
CUATRECASAS, P
中科院分区：
文献类型：
--
作者：
NIEDEL, JE;KAHANE, I;CUATRECASAS, P
四甲基罗丹明标记的 N-甲酰基-Nle-Leu-Phe-Nle-Tyr-Lys 是人类中性粒细胞的有效化学引诱剂。通过视频增强显微镜观察该肽与活中性粒细胞的结合。在 37°C 时，首先看到漫射膜荧光，随后荧光肽快速聚集和内化。这些过程依赖于与甲酰肽趋化受体的特异性结合。
Tetramethylrhodamine labeledN-formyl-Nle-Leu-Phe-Nle-Tyr-Lys is a potent chemoattractant for human neutrophils. Binding of this peptide to living neutrophils was observed by means of video intensification microscopy. At 37°C, diffuse membrane fluorescence was seen initially, followed by rapid aggregation and internalization of the fluorescent peptide. These processes are dependent on specific binding to the formyl peptide chemotactic receptor.