The Windblown: Possible Explanations for Dinophyte DNA in Forest Soils
The Windblown: Possible Explanations for Dinophyte DNA in Forest Soils
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DOI：
10.1111/jeu.12833
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发表时间：
2020-12-10
影响因子：
2.2
通讯作者：
Dunthorn, Micah
中科院分区：
文献类型：
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作者：
Gottschling, Marc;Czech, Lucas;Dunthorn, Micah
Dinophytes广泛分布在海洋和淡水中，但在陆地环境中还没有结论性的记录。在这里，我们从新热带雨林土壤的环境DNA元条形码数据集中评估了这些原生生物的存在。利用参考比对和树的系统发育定位方法，我们发现在系统发育上被定位为恐龙的许多测序读数与分类分配、环境偏好、营养模式或休眠无关。土壤中的所有苔藓植物都是水生物种的风传播单位，而不是陆地环境的生物活跃居民。
Dinophytes are widely distributed in marine- and fresh-waters, but have yet to be conclusively documented in terrestrial environments. Here, we evaluated the presence of these protists from an environmental DNA metabarcoding dataset of Neotropical rainforest soils. Using a phylogenetic placement approach with a reference alignment and tree, we showed that the numerous sequencing reads that were phylogenetically placed as dinophytes did not correlate with taxonomic assignment, environmental preference, nutritional mode, or dormancy. All the dinophytes in the soils are rather windblown dispersal units of aquatic species and are not biologically active residents of terrestrial environments.