Properties of the new fluorescent Na+ indicator CoroNa Green:: Comparison with SBFI and confocal Na+ imaging
Properties of the new fluorescent Na+ indicator CoroNa Green:: Comparison with SBFI and confocal Na+ imaging
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DOI：
10.1016/j.jneumeth.2006.01.009
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发表时间：
2006-09-15
影响因子：
3
通讯作者：
Rose, Christine R.
中科院分区：
文献类型：
--
作者：
Meier, Silke D.;Kovalchuk, Yury;Rose, Christine R.
神经元活动在细细胞过程如树突和棘中引起大量Na+瞬变。这种Na+瞬变的生理后果在很大程度上仍然是未知的。高分辨率Na+成像是研究这些问题的关键，到目前为止，使用荧光Na+指示剂结合苯并呋喃三乙酸钠（SBFI）的双光子成像一直是首选方法。最近，一种新的Na+指示剂染料CoroNa绿色（CoroNa），其在492 nm处具有最大吸光度，已经变得可用。在本研究中，我们比较了SBFI与CoroNa的性质，通过在海马脑片神经元中进行Na+测量。我们表明，CoroNa是合适的测量Na+瞬变使用非共焦宽场成像与CCD相机。然而，当与SBFI相比时，大量跨膜染料泄漏和较低的Na+灵敏度明显是缺点。我们还使用共聚焦激光扫描系统测试了CoroNa对Na+瞬变高分辨率成像的适用性。我们证明，CoroNa，在对比SBFI，可以采用共聚焦成像使用传统的氩激光器和报告的第一个Na+测量使用这种染料在树突。总之，CoroNa可能被证明是一个有价值的工具，共聚焦Na+成像在精细的细胞过程。(c)2006 Elsevier B.V.保留所有权利。
Neuronal activity causes substantial Na+ transients in fine cellular processes such as dendrites and spines. The physiological consequences of such Na+ transients are still largely unknown. High-resolution Na+ imaging is pivotal to study these questions, and, up to now, two-photon imaging with the fluorescent Na+ indicator sodium-binding benzofuran isophthalate (SBFI) has been the primary method of choice. Recently, a new Na+ indicator dye, CoroNa Green (CoroNa), that has its absorbance maximum at 492 nm, has become available. In the present study, we have compared the properties of SBFI with those of CoroNa by performing Na+ measurements in neurons of hippocampal slices. We show that CoroNa is suitable for measurement of Na+ transients using non-confocal wide-field imaging with a CCD camera. However, substantial transmembrane dye leakage and lower Na+ sensitivity are clearly disadvantages when compared to SBFI. We also tested CoroNa for its suitability for high-resolution imaging of Na+ transients using a confocal laser scanning system. We demonstrate that CoroNa, in contrast to SBFI, can be employed for confocal imaging using a conventional argon laser and report the first Na+ measurements in dendrites using this dye. In conclusion, CoroNa may prove to be a valuable tool for confocal Na+ imaging in fine cellular processes. (c) 2006 Elsevier B.V. All rights reserved.